Development of Objective Methods for Fluorosis Diagnosis

氟中毒诊断客观方法的发展

• 批准号: 7028356
• 负责人: ESPERANZA A MARTINEZ-MIER
• 金额: $ 22.19万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7028356
• 关键词: adolescence (12-20); bioimaging /biomedical imaging; clinical research; dental visualization; diagnosis design /evaluation; digital imaging; fluorimetry; fluorosis; human subject; image processing; middle childhood (6-11); photography; therapy adverse effect

DESCRIPTION: Methods currently used to diagnose Dental fluorosis rely on subjective measurements based on epidemiological indices. This has resulted in absence of diagnostic sensitivity, specificity and has led to inconsistencies in data summary and report. The proposed investigation Aims at developing objective and quantitative methods for fluorosis diagnosis using imaging technology (Quantitative Light Fluorescence [QLF] and digital intraoral photography) and visual examination. Validation of results will be provided by direct microanalysis of fluoride content, histological examination and 3-d quantification of mineral content of fluoride-related hypomineralizations. Both extracted teeth and children ages 7 to 13 will be examined as part of the study. Identification of selected surfaces as fluorosis cases, caries lesions or sound/non-fluorosis cases will be performed visually and will be recorded with QLF and digital intraoral photographs. Quantification of QLF images will be conducted. Methods to quantify photographic images using image analysis software will be developed. A method to quantify fluoride-related hypomineralizations using micro CT will be also developed. Sensitivity, specificity and repeatability of each method will be assessed. The development of objective methods for Dental fluorosis diagnosis will improve Dental fluorosis and fluoride research. The proposed investigation will provide the opportunity to identify such methods for the assessment of Dental fluorosis. It will provide the methodological framework to resolve discrepancies that currently make it difficult to compare results of studies dealing with the epidemiology, and the biological and esthetic effects of Dental fluorosis.

描述：目前用于诊断氟牙症的方法依赖于基于流行病学指标的主观测量。这导致缺乏诊断敏感性和特异性，并导致数据总结和报告不一致。本研究旨在利用影像学技术（定量光荧光（quantitative Light Fluorescence， QLF）和数字口内摄影）和目视检查，建立客观、定量的氟中毒诊断方法。结果的验证将通过氟化物含量的直接微量分析、组织学检查和与氟化物有关的低矿化矿物含量的三维量化来提供。作为研究的一部分，拔掉的牙齿和7至13岁的儿童都将接受检查。对选定的表面进行氟中毒病例、龋齿病变或声音/非氟中毒病例的识别，并将用QLF和数字口内照片进行记录。将对QLF图像进行定量分析。将开发使用图像分析软件量化摄影图像的方法。还将开发一种利用微型CT定量氟化物相关低矿化的方法。评估每种方法的敏感性、特异性和可重复性。发展客观的氟牙症诊断方法将促进氟牙症和氟化物的研究。拟议的调查将为确定评估氟斑牙的方法提供机会。它将提供方法框架，以解决目前难以比较有关氟斑牙的流行病学以及生物学和美学影响的研究结果的差异。