Gold standard methods for fluoride analysis

描述(由申请人提供)：氟化物被广泛认为可以降低龋齿的患病率。因此，无论是旨在增强其有益影响的研究，还是旨在减少其有害影响的研究，都仍然是相关的。这类研究可以受益于氟化物分析技术的改进。此外，只有在有测量氟化物水平、摄入量和浓度的标准化方法的情况下，才能在这一领域进行有意义的国际研究。目前，现有的氟测定技术还不规范，还没有建立一个通用的氟测定标准。虽然有各种各样的技术可用，但没有一种被普遍接受。目前的项目旨在与现有的分析实验室合作，开发分析不同类型样品中氟化物的全球标准方法。 为了开发这些标准的全球氟化物分析方法，口腔健康研究所的氟化物实验室建议与目前正在进行氟化物分析的另外六个完善的实验室合作。拟议的合作将包括使用每个实验室目前使用的方法分析一系列180个生物和环境样本的氟含量。这些分析的结果和所用分析方法的说明将从每个实验室收集并分发给所有合作者。然后将制定一项计划，以解决结果中的任何差异，并在口腔健康研究所的所有合作者会议上确定首选的分析方法。然后，将由所有实验室使用首选的分析方法对另一系列生物样本进行分析，最后将编写一份科学论文，审查收集的数据和更好的分析方法，以便出版。 拟议的调查将为七个经认可的实验室提供合作机会，以便就详细的协议达成共识，这些协议将确定和描述特定类型样品的氟化物分析的黄金标准技术。拟议的研究将提供一个机会，以解决目前难以比较涉及氟化物分析的许多研究的结果的差异。

DESCRIPTION (provided by applicant): Fluoride is widely recognized for reducing the prevalence of Dental caries. Therefore, either studies aimed to enhance its beneficial effects or to reduce its detrimental effects continues to be relevant. Such studies can benefit from improvements in the techniques employed for the analysis of fluoride. In addition, meaningful international studies in this field can only be conducted if standardized methods for measuring the levels, ingestion and concentrations of fluoride, are available. Currently available fluoride measurement techniques are not standardized and a universal standard for fluoride determination has not been established. Although a variety of techniques are available, none have been accepted for universal use. The current project Aims to collaborate with established analytical laboratories to develop standard, global methods for analyzing fluoride in different types of samples. In order to develop these standard, global methods for fluoride analysis, the Fluoride Laboratory at the Oral Health Research Institute proposes to collaborate with six, additional, well-established laboratories that are currently conducting fluoride analyses. The proposed collaboration will include the analysis of the fluoride content of a series of 180 biological and environmental samples using the methods in current use in each laboratory. Results of these analyses and descriptions of the analytical procedures used will be collected and distributed from each laboratory to all collaborators. A plan will be then developed to resolve any differences in results and to identify preferred analytical procedures in a meeting of all collaborators at the Oral Health Research Institute. Analyses of another series of biological samples by all laboratories using the preferred analytical methods will be then conducted and, finally a scientific paper reviewing the data collected and the preferable analytical procedures will be developed for publication. The proposed investigation will provide an opportunity for seven recognized laboratories to collaborate in order to reach consensus about detailed protocols that will identify and describe gold standard techniques for fluoride analysis of specific types of samples. The proposed study will provide an opportunity to resolve discrepancies that currently make it difficult to compare the results of the many studies dealing with fluoride analyses.