Cell and Molecular Biology of Marine Elasmobranchs

海洋软骨鱼的细胞和分子生物学

• 批准号: 6931225
• 负责人: DAVID William BARNES
• 金额: $ 36.56万
• 依托单位: MOUNT DESERT ISLAND BIOLOGICAL LAB
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6931225
• 关键词: Chondrichthyes; biological models; brain cell; cell biology; cell line; cell proliferation; embryo /fetus tissue /cell culture; gene expression; kidney cell; liver; model design /development; molecular biology; technology /technique development; transfection /expression vector

DESCRIPTION (provided by applicant): This proposal responds to the Program "Development of Non-mammalian Models and Related Biological Materials for Research." Specifically, the program announcement mentions "Species such as fishes...and cell lines and cell culture models for non-mammalian animals where culture systems are not well developed but would be widely used." We propose to develop cell lines from marine elasmobranchs (sharks and skates), animals that are broadly exploited in biomedical research as models in physiology, endocrinology, toxicology and genomics. Currently there are no homologous in vitro systems by which investigators using these models can test hypotheses or confirm predictions at a molecular level, and application of new technologies is needed. The premise driving this project is that in vitro culture of specialized cell types can be accomplished by taking non-conventional approaches, including removal or reduction of serurnas a medium component and replacement with cell type-specific supplements, such as peptide growth factors. We have used this approach to generate cell lines from zebrafish, pufferfish (fugu), and other fish species. This work will take advantage of the unique resources of the Mount Desert Island Biological Laboratory, a research facility dedicated to the study of marine organisms to discover biological mechanisms of significance to human health, and world center for the use of the dogfish shark (Squalus acanthias) and little skate (Raja erinacea) as research models. We propose to develop differentiation-competent, continuously proliferating cell lines from (1) liver, brain, kidney and salt (rectal) gland of the dogfish shark, and (2) liver and embryo of the little skate. We also propose to (3) develop systems for DNA transfection and plasmid-derived gene expression in these cell lines. Success will provide a platform for original and innovative experiments, accelerating the development of concepts driving multiple areas of biomedical research addressed by the large number of investigators using these models.

描述（由申请人提供）：本提案响应“非哺乳动物模型和相关生物材料的研究开发”项目。具体来说，节目公告提到了“鱼类等物种……培养系统不发达的非哺乳动物的细胞系和细胞培养模型将得到广泛应用。”我们建议从海洋板鳃动物（鲨鱼和冰鱼）中培养细胞系，这些动物在生物医学研究中被广泛利用，作为生理学、内分泌学、毒理学和基因组学的模型。目前还没有研究人员利用这些模型在分子水平上验证假设或证实预测的同源体外系统，需要新技术的应用。推动这个项目的前提是，可以通过非传统的方法来完成特化细胞类型的体外培养，包括去除或减少浆液作为培养基成分，并用细胞类型特异性补充剂替代，如肽生长因子。我们已经用这种方法从斑马鱼、河豚和其他鱼类中产生细胞系。这项工作将利用Mount Desert Island生物实验室的独特资源，该实验室是一个致力于研究海洋生物以发现对人类健康有重要意义的生物机制的研究设施，也是世界使用角鲨鲨（Squalus acanthias）和小鳐（Raja erinacea）作为研究模型的中心。我们建议从角鲨的肝脏、大脑、肾脏和盐（直肠）腺，以及小鳐的肝脏和胚胎中培养具有分化能力、持续增殖的细胞系。我们还建议(3)在这些细胞系中开发DNA转染和质粒衍生基因表达系统。成功将为原创和创新实验提供一个平台，加速概念的发展，推动生物医学研究的多个领域，大量研究者使用这些模型进行研究。