Plasmonic Fluorescence Microarray Detection System

等离子荧光微阵列检测系统

• 批准号: 6991758
• 负责人: Salvador M. Fernandez
• 金额: $ 12.25万
• 依托单位: CIENCIA, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-05 至 2006-03-04
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6991758
• 关键词: charge coupled device camera; fluorescence; fluorescent dye /probe; ionophores; microarray technology; microprocessor /microchip; surface plasmon resonance; technology /technique development

DESCRIPTION (provided by applicant): The long term objective of the proposed project I is to develop an affordable, highly parallel and highly sensitive detection system for protein and DMA chip applications. This system is based on a novel fluorescence detection technology with the potential for 10- to 1000-fold higher sensitivity than current state of- the-art fluorescence imaging systems. Importantly, the proposed technology will be implemented with relatively inexpensive CCD imaging instrumentation and with low-cost disposable chips. The principle behind the proposed enhancement in fluorescence detection sensitivity is a phenomenon known as surface plasmon coupled fluorescence (SPCF). The basis for the proposed technology rests on two key elements: a metal-coated, plastic sensor chip with periodic surface features that enable resonant coupling of optical radiation into surface plasmons, and surface chemistry with an interfacial architecture that enables positioning of the detection fluorescent dyes (e.g., fluorescent antibody conjugates) within 10-200nm of the metal surface. With this architecture and under appropriate excitations conditions, fluorescence emission is modified in two striking ways. First, the excitation field is enhanced, and second emission is highly directional, which can vastly improved optical collection efficiency. The specific aims of the Phase I are to 1) set up an experimental apparatus to test the proposed concept, 2) to fabricate chips with the appropriate interfacial architecture, and 3) to characterize the performance of the system. Expected future products (instrument and disposable chips) will be initially targeted to the analytical microchip market, the fastest growing technology segment in the life sciences today. The range of applications is very broad, covering fields as diverse as pharmaceutical development, food testing, clinical diagnostics, forensics, environmental analysis, bioprocess monitoring and biodefense.

描述(由申请人提供)：拟议项目I的长期目标是为蛋白质和DMA芯片应用开发一种负担得起的、高度并行和高灵敏度的检测系统。该系统基于一种新的荧光检测技术，其灵敏度可能比目前最先进的荧光成像系统高10到1000倍。重要的是，拟议的技术将使用相对便宜的ccd成像仪器和低成本的一次性芯片来实施。所提出的增强荧光检测灵敏度的原理是一种被称为表面等离子体耦合荧光(SPCF)的现象。拟议技术的基础依赖于两个关键元素：具有周期性表面特征的金属涂层塑料传感器芯片，使光辐射能够与表面等离子体共振耦合，以及具有界面结构的表面化学，使检测荧光染料(例如荧光抗体结合物)能够定位在金属表面10-200 nm范围内。在这种结构下，在适当的激发条件下，荧光发射以两种引人注目的方式进行了修改。第一，激发场增强，第二次发射具有高方向性，可以极大地提高光收集效率。第一阶段的具体目标是1)建立一个实验装置来测试所提出的概念，2)制造具有适当界面结构的芯片，以及3)表征系统的性能。 预计未来的产品(仪器和一次性芯片)将首先瞄准分析微芯片市场，这是当今生命科学中增长最快的技术领域。应用范围非常广泛，涵盖制药开发、食品检测、临床诊断、法医学、环境分析、生物过程监测和生物防御等多个领域。