System for Multiplex Real-Time Analysis of Nucleic Acids

核酸多重实时分析系统

• 批准号: 6935717
• 负责人: Salvador M. Fernandez
• 金额: $ 17.1万
• 依托单位: CIENCIA, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-20 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6935717
• 关键词: biohazard detection; biotechnology; gene expression; genetic polymorphism; microarray technology; molecular /cellular imaging; nucleic acid sequence; polymerase chain reaction; technology /technique development; temperature

DESCRIPTION (provided by applicant): The objective of this Phase I project is to test the feasibility of biochip imaging system which will vary the temperature of the arrays and perform real-time imaging and quantification during PCR and probe meltoff. A specialized microarray of discrete hydroge! Compartments for array-based PCR amplification will be tested with the prototype instrument. The technology is expected to be appropriate for significant applications such as gene expression, polymorphism scoring and microbial detection and typing. Consumption of sample and reagents will be minimized, because significant multiplex factors can be realized in small volumes. The expected outcome of this project is the specification of a versatile instrument for research or diagnostics that could be brought into the development phase.

描述（由申请人提供）： 该项目的第一阶段的目标是测试生物芯片成像系统的可行性，该系统将改变阵列的温度，并在PCR和探针熔化期间执行实时成像和定量。一种特殊的离散水微阵列！将使用原型仪器测试基于阵列的PCR扩增的兼容性。预计该技术将适用于基因表达、多态性评分和微生物检测和分型等重要应用。样品和试剂的消耗将最小化，因为可以在小体积中实现重要的多重因子。该项目的预期成果是确定可进入开发阶段的研究或诊断用多功能仪器的规格。