Grating-Coupled Evanescent Wave Sensor

光栅耦合倏逝波传感器

• 批准号: 6485840
• 负责人: Salvador M. Fernandez
• 金额: $ 12.3万
• 依托单位: CIENCIA, INC.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2002-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6485840
• 关键词: intermolecular interaction; ligands; metal complex; method development; microarray technology; protein binding

The goal of this effort is to develop a novel label-free detection technology for application to protein microarrays. The objective of the Phase I is to build a breadboard prototype to characterize the performance of this new approach and to establish feasibility. The technology is based on a multilayer grating-coupled (MGR) evanescent wave sensing method with the potential to provide highly multiplexed measurements for massively parallel analysis of microarrays with high sensitivity and without the drawbacks and limitations of label-based methods or other label-free methods such as surface plasmon resonance (SPR). This innovation addresses the "detection problem" in proteomics; that is, the difficult and pressing problem of how to accurately measure binding to a protein microarray with high sensitivity and high spatial and temporal resolution. We will build a prototype imaging system and demonstrate multiplexed measurements with prototype microarrays made on disposable sensor chips. Preliminary results provide a solid foundation for the proposed work and indicate a high probability of success. Potential applications range from basic research to drug target discovery to clinical diagnostics. Examples include protein expression profiling; high-throughput ligand fishing, identifying new receptor and natural ligands, immune response characterization, homology studies on protein families, identifying disease markers and others. We believe that the field of drug discovery and development presents one of the largest immediate commercial opportunities for the proposed technology, followed by clinical diagnostics in the longer term. We anticipate that the high-throughput, label-free microarray-based MGR system proposed here will capture a significant segment of these market. PROPOSED COMMERCIAL APPLICATION: Potential applications range from basic research to drug target discovery to clinical diagnostics. Specific examples include protein expression profiling; high-throughput annotation of gene products, defining binding domains, epitopes and interacting molecular motifs, high-throughput ligand fishing, identifying new receptors and natural ligands, immune response characterization, homology studies on protein families, identifying disease markers and others.

这项工作的目标是开发一种新的无标记检测技术，应用于蛋白质微阵列。第一阶段的目标是建立一个面包板原型，以表征这一新方法的性能并确定其可行性。该技术基于多层光栅耦合(MGR)逝去波传感方法，有可能为大规模并行分析微阵列提供高度多路复用的测量，具有高灵敏度，并且没有基于标记的方法或其他非标记方法(如表面等离子体共振(SPR))的缺点和限制。这一创新解决了蛋白质组学中的“检测问题”，即如何以高灵敏度和高时空分辨率准确测量与蛋白质微阵列的结合这一困难而紧迫的问题。我们将建立一个原型成像系统，并用一次性传感器芯片制成的原型微阵列演示多路测量。初步结果为拟议的工作提供了坚实的基础，并表明成功的可能性很高。潜在的应用范围从基础研究到药物靶点发现到临床诊断。例如，蛋白质表达谱分析、高通量配基捕捞、识别新的受体和天然配基、免疫反应表征、蛋白质家族同源性研究、识别疾病标记物等。我们认为，药物发现和开发领域是拟议技术最大的直接商业机会之一，其次是长期的临床诊断。我们预计，这里提出的高通量、基于无标记微阵列的MGR系统将占据这些市场的很大一部分。 建议的商业应用： 潜在的应用范围从基础研究到药物靶点发现到临床诊断。具体的例子包括：蛋白质表达谱分析；基因产品的高通量注释；确定结合区域、表位和相互作用的分子基序；高通量配体捕捞；确定新的受体和天然配体；免疫反应特征；蛋白质家族的同源性研究；确定疾病标记物等。