Raman Monitor for Diagnosis of Salt Sensitivity

描述（由申请人提供）：拟议项目的重点是评估一种新的测试方法在常规体检期间识别盐敏感个体的可行性。我们计划利用的技术是表面增强拉曼散射（Sers），这是一种用于精确实验室鉴定有机化合物的众所周知的分析技术。我们建议通过Sers拉曼光谱分析来确定尿液中盐敏感性的化学标志物。总体目标是开发一种便携式现场设备，其足够通用，使得其可以用于立即识别盐敏感个体中的化学标记物，预期在尿液中作为痕量存在的标记物，来自在身体检查过程中现场收集的样品，以允许及时进行适当的干预和/或治疗。 我们修订的第1阶段项目旨在评估拉曼定量一系列盐敏感性代表性标志物水平的能力，该标志物水平存在于具有不同浓度的选定代表性标志物材料的简单模型尿样中。将对实际尿液样本进行额外的初始检测，以首次评价拉曼监测方法的灵敏度和选择性。在第2阶段，我们将扩大过程，现场尿液分析平行实际现场检查和临床试验程序。

DESCRIPTION (provided by applicant): The focus of the proposed program is to evaluate the feasibility of a new testing approach for the identification of individuals with salt sensitivity during routine physical examination. The technology we plan to utilize is surface-enhanced Raman scattering (SERS), a well-known analytical technique for precise lab identification of organic compounds. We propose to identify the chemical markers for salt sensitivity excreted in the urine by SERS Raman spectroscopy analysis. The overall aim is to develop a portable on-site device versatile enough so that it can be used for immediate identification of chemical markers in salt sensitive individuals, markers expected to be present as trace amounts in urine, from samples collectible on-site in the course of a physical examination to allow appropriate intervention and/or treatment in a timely fashion. Our revised Phase 1 program is designed to assess the capability of Raman to quantify the levels of a range of representative markers for salt sensitivity in a simple model urine sample with varying concentrations of selected representative marker materials present. Additional initial testing will be performed on actual urine samples for a first evaluation of the sensitivity and selectivity of the Raman monitoring approach. In Phase 2 we would expand the process to on-site urinalysis paralleling actual on-site examination and clinical testing procedures.