Raman Monitor for Diagnosis of Salt Sensitivity

用于诊断盐敏感性的拉曼监测仪

• 批准号: 6933716
• 负责人: Richard H Clarke
• 金额: $ 10万
• 依托单位: RAMAN SYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2005-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6933716
• 关键词: Raman spectrometry; albumins; aldosterone; biomedical equipment development; clinical research; diagnosis design /evaluation; diagnostic tests; dietary excess; dietary sodium; human tissue; portable biomedical equipment; urinalysis

DESCRIPTION (provided by applicant): The focus of the proposed program is to evaluate the feasibility of a new testing approach for the identification of individuals with salt sensitivity during routine physical examination. The technology we plan to utilize is surface-enhanced Raman scattering (SERS), a well-known analytical technique for precise lab identification of organic compounds. We propose to identify the chemical markers for salt sensitivity excreted in the urine by SERS Raman spectroscopy analysis. The overall aim is to develop a portable on-site device versatile enough so that it can be used for immediate identification of chemical markers in salt sensitive individuals, markers expected to be present as trace amounts in urine, from samples collectible on-site in the course of a physical examination to allow appropriate intervention and/or treatment in a timely fashion. Our revised Phase 1 program is designed to assess the capability of Raman to quantify the levels of a range of representative markers for salt sensitivity in a simple model urine sample with varying concentrations of selected representative marker materials present. Additional initial testing will be performed on actual urine samples for a first evaluation of the sensitivity and selectivity of the Raman monitoring approach. In Phase 2 we would expand the process to on-site urinalysis paralleling actual on-site examination and clinical testing procedures.

描述(由申请人提供)：该计划的重点是评估一种新的测试方法的可行性，以在常规体检中识别盐敏感者。我们计划使用的技术是表面增强拉曼散射(SERS)，这是一种著名的分析技术，用于在实验室精确识别有机化合物。我们建议通过SERS拉曼光谱分析来鉴定尿液中盐敏感性的化学标志物。总体目标是开发一种具有足够多用途的便携式现场设备，以便它能够用于从在体检过程中现场收集的样本中立即识别盐敏感个体的化学标记，即预计在尿液中以痕量存在的标记，以便及时进行适当的干预和/或治疗。 我们修订的第一阶段计划旨在评估拉曼技术在一个简单的模型尿样中量化一系列盐敏感性代表性标志物水平的能力，其中存在不同浓度的选定代表性标志物材料。将对实际尿样进行额外的初步测试，以首次评估拉曼监测方法的灵敏度和选择性。在第二阶段，我们将把这一过程扩展到现场尿液分析，与实际的现场检查和临床测试程序并行。