Monitor for Multiple Biomarkers for Detection of Alcohol Consumption

监测多种生物标志物以检测酒精消耗量

• 批准号: 8591345
• 负责人: Richard H Clarke
• 金额: $ 14.98万
• 依托单位: YELLOWSTONE SCIENTIFIC INSTRUMENTS
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-15 至 2014-12-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8591345
• 关键词: Alcohol consumption; Alcohols; Biological Markers; Blood; Chemistry; Clinical; Detection; Development; Devices; Early Diagnosis; Gold; High Pressure Liquid Chromatography; Laboratories; Lasers; Liquid Chromatography; Mass Spectrum Analysis; Measurement; Measures; Modeling; Monitor; Organ; Persons; Phase; Plasma; Plastics; Populations at Risk; Raman Spectrum Analysis; Research; Resources; Saliva; Sampling; Serum; Site; Source; Spectrum Analysis; Surface; Techniques; Technology; Testing; Time; Tissues; Update; Urine; alcohol exposure; analytical method; base; clinical research site; cost effective; detector; diethyl sulfate; light scattering; nanoparticle; phosphatidylethanol; programs; public health relevance; response; saliva analysis; surface coating; wasting

DESCRIPTION: We propose to develop a new type of monitor to directly and simultaneously identify multiple biomarker metabolites in persons suspected of having been exposed to alcohol. The technology we plan to utilize is surface-enhanced Raman scattering (SERS), based on a well-known inelastic laser light scattering analytical technique for precise lab identification of organic compounds. Biomarker detectors and biomarker-based tests must be sensitive, reproducible, affordable, transportable, reliable, and the samples employed in the detectors must be easy to collect and obtain from serum, plasma, urine, saliva, or other sources. The ideal detection device in both cases is one that is small, portable, easy to use and capable of rapid analysis of multiple biomarkers in a clinical setting, with a minimum of false positives and false negatives to avoid wasting resources and missing actual exposure problems. By detecting the presence of the alcohol biomarkers promptly and at emerging concentrations, the alcohol threat to tissue and internal organs may be identified early in the exposure cycle, expanding treatment options in the population at risk. The proposed research program seeks to utilize SERS spectroscopy for rapid at-site blood, urine or saliva analysis for multiple biomarkers in a single, field-deployable device, bringing the power of the Raman technique in a practical, cost-effective configuration to at-site clinical analysis.

描述：我们建议开发一种新型的监测仪，以直接和同时识别多种生物标志物代谢物在怀疑已经暴露于酒精的人。我们计划利用的技术是表面增强拉曼散射（SERS），基于一种众所周知的非弹性激光散射分析技术，用于精确的实验室鉴定有机化合物。生物标志物检测器和基于生物标志物的检测必须灵敏、可重复、可负担、可运输、可靠，并且检测器中使用的样品必须易于从血清、血浆、尿液、唾液或其他来源中收集和获得。在这两种情况下，理想的检测设备都是体积小、便携、易于使用、能够在临床环境中快速分析多种生物标志物的设备，假阳性和假阴性最少，以避免浪费资源和遗漏实际暴露问题。通过及时检测酒精生物标志物的存在和新浓度，可以在暴露周期的早期识别酒精对组织和内脏的威胁，从而扩大高危人群的治疗选择。拟议的研究计划旨在利用SERS光谱在单个现场可部署的设备中对多种生物标志物进行快速现场血液、尿液或唾液分析，将拉曼技术的力量以实用、经济的方式配置到现场临床分析中。