Monitor for Multiple Biomarkers for Detection of Alcohol Consumption

监测多种生物标志物以检测酒精消耗量

• 批准号: 9144294
• 负责人: Richard H Clarke
• 金额: $ 44.27万
• 依托单位: YELLOWSTONE SCIENTIFIC INSTRUMENTS
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-15 至 2018-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9144294
• 关键词: Alcohol consumption; Alcoholism; Alcohols; Animal Model; Biological Markers; Blood; Blood specimen; Chemistry; Clinical; Detection; Development; Devices; Early Diagnosis; Ethanol; Gold; Health; High Pressure Liquid Chromatography; Human; Incubated; Laboratories; Lasers; Liquid Chromatography; Mass Spectrum Analysis; Measurement; Measures; Monitor; National Institute on Alcohol Abuse and Alcoholism; Organ; Patients; Persons; Phase; Populations at Risk; Procedures; Raman Spectrum Analysis; Reader; Research; Resources; Sampling; Site; Source; Spectrum Analysis; Surface; Techniques; Technology; Testing; Tissues; Update; Urine; alcohol exposure; analytical method; base; clinical research site; cost effective; detector; diethyl sulfate; in vivo; interest; light scattering; nanoparticle; phosphatidylethanol; problem drinker; programs; prototype; repository; response; surface coating; wasting

 DESCRIPTION (provided by applicant): We propose to develop a new type of monitor to directly and simultaneously identify multiple biomarker metabolites in persons suspected of having been exposed to alcohol. The technology we plan to utilize is surface-enhanced Raman scattering (SERS), based on a well-known inelastic laser light scattering analytical technique for precise lab identification of organic compounds. Biomarker detectors and biomarker-based tests must be sensitive, reproducible, affordable, transportable, reliable, and the samples employed in the detectors must be easy to obtain from sources on-site. The ideal detection device in both cases is one that is small, portable, easy to use and capable of rapid analysis of multiple biomarkers in a clinical setting, with a minimum of false positives and false negatives to avoid wasting resources and missing actual exposure problems. By detecting the presence of the alcohol biomarkers promptly and at emerging concentrations, the alcohol threat to tissue and internal organs may be identified early in the exposure cycle, expanding treatment options in the population at risk. The proposed research program seeks to utilize SERS spectroscopy for rapid at-site blood and urine analysis for multiple biomarkers in a single, field-deployable device, bringing the power of the Raman technique in a practical, cost-effective configuration to at-site clinical analysis.

 描述（由申请人提供）：我们建议开发一种新型监测器，以直接和同时识别疑似暴露于酒精的人体内的多种生物标志物代谢物。我们计划利用的技术是表面增强拉曼散射（Sers），基于一种众所周知的非弹性激光散射分析技术，用于精确的实验室鉴定有机化合物。生物标志物检测器和基于生物标志物的测试必须灵敏、可再现、价格合理、可运输、可靠，并且检测器中使用的样品必须易于从现场来源获得。在这两种情况下，理想的检测设备都是小型、便携、易于使用并且能够在临床环境中快速分析多种生物标志物的设备，具有最小的假阳性和假阴性，以避免浪费资源和错过实际暴露问题。通过及时检测酒精生物标志物的存在和新出现的浓度，可以在暴露周期的早期确定酒精对组织和内脏的威胁，扩大风险人群的治疗选择。拟议的研究计划旨在利用Sers光谱在一个单一的现场部署设备中快速现场血液和尿液分析多种生物标志物，将拉曼技术的力量以实用，具有成本效益的配置带到现场临床分析中。