A Nanoscale Evanescent Wave Magnetic Resonance Probe

纳米级倏逝波磁共振探头

• 批准号: 7120161
• 负责人: HAITAO YANG
• 金额: $ 19.99万
• 依托单位: INTEMATIX CORPORATION
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-05-15 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7120161
• 关键词: atomic force microscopy; electron spin resonance spectroscopy; fluorescent dye /probe; magnetic resonance imaging; microwave spectrometry; nanotechnology; technology /technique development; three dimensional imaging /topography

DESCRIPTION (provided by applicant): This proposed research strives to develop a novel high-resolution and high-sensitivity evanescent wave magnetic resonance probe for molecular biomedical research. Conventional NMR technique can determine molecular structure of large ensemble of homogenous molecules through precise measurement of chemical shift of nuclear spin resonance in a uniform magnetic field, while imaging technique, MRI, lost this critical capability due to its high field gradient needed for imaging. We propose here to develop novel magnetic resonance detection technology with optimized sensitivity and resolution. Through two-phase R&D, our ultimate goal is to demonstrate magnetic resonance detection of single biological molecules with spatial resolution of 10 nm. The proposed technology, an Evanescence-Wave Magnetic Resonance Probe (EWMRP), integrates many aspects of well-established magnetic resonance technology with a novel evanescence-wave (microwave or RF) emission/detection device and a novel atomic-resolution force microscopy element. The spatially resolved spin resonance is realized through evanescent wave generated at the probe tip rather than magnetic field gradient. Therefore, this technology offers the capability of probing precise chemical shifts of differently situated nuclei locally. The proposed EWMR probe will be able to provide simultaneously high spatial resolution, high contrast topography and high sensitivity spin magnetic resonance spectroscopy, which is expected to have profound implications for in situ study of biochemical processes of a single molecule (e.g., a protein) and its sub-structures, on a surface or inside a cell.

描述(申请人提供)：这项拟议的研究致力于开发一种新型的高分辨率和高灵敏度的逝去波磁共振探针，用于分子生物医学研究。传统的核磁共振技术可以通过精确测量均匀磁场中核磁共振的化学位移来确定大分子系综的分子结构，而核磁共振成像技术由于其成像所需的高磁场梯度而失去了这一关键能力。我们建议开发具有最佳灵敏度和分辨率的新型磁共振检测技术。通过两个阶段的研发，我们的最终目标是展示空间分辨率为10 nm的单个生物分子的磁共振检测。所提出的技术，即消失波磁共振探头(EWMRP)，将成熟的磁共振技术的许多方面与新的消失波(微波或射频)发射/检测装置和新的原子分辨率力显微镜元件相结合。空间分辨自旋共振是通过在探针尖端产生的逝去波实现的，而不是通过磁场梯度实现的。因此，这项技术提供了探测不同位置原子核局部精确化学位移的能力。所提出的EWMR探针将能够同时提供高空间分辨率、高对比度的地形和高灵敏度的自旋磁共振波谱，这有望对原位研究单个分子(例如蛋白质)及其亚结构的生化过程、表面或细胞内的生物化学过程产生深远的影响。