SLEEP DEPRIVATION AND 5-HT AUTOREGULATORY PROCESSES

睡眠剥夺和 5-HT 自动调节过程

• 批准号: 6837211
• 负责人: Russell E. Poland
• 金额: --
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-02-12 至 2005-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6837211
• 关键词: depression; enzyme activity; gene expression; laboratory rat; neurochemistry; neurotransmitter biosynthesis; serotonin; serotonin receptor; sleep deprivation; tryptophan; tryptophan 5 monooxygenase

DESCRIPTION (applicant's abstract): This Mentored Clinical Scientist Developmental Award application includes a program of career developmental and research training for the applicant, Faustino Lopez-Rodriguez, M.D, Ph.D. The ultimate goal of the applicant is to develop as an independent investigator in examining the neurobiology of antidepressant mechanisms of action. The career developmental and research plan are designed to allow the applicant to reach this goal. The applicant intends to supplement his background in electrophysiology with techniques in neurochemistry (microdialysis of biogenic monoamines in behaving animals and high performance liquid chromatography) and molecular biology, which he plans to acquire during the Award period. The applicant intends to apply his expertise in sleep physiology to study the neurobiological mechanisms underlying the antidepressant effects induced by sleep deprivation. In this proposal, and as a first step in an overall research program, the applicant will concentrate on the effects of sleep deprivation on serotonin (5-HT) activity. His specific aims are as follows. 1) Sleep deprivation induces changes in 5-HT release in forebrain structures and is accompanied by sustained high levels of 5-HT in the raphe. 2) Sleep deprivation changes the activity of 5-HT1A and 5-HT1B autoreceptors. The applicant hypothesizes that sleep deprivation blocks the effects of 5-HT1A and/or 5HT1B agonists on 5-HT release, as well as the amounts and synthesis of 5-HT1A and 5-HT1B autoreceptors. 3) Sleep deprivation modifies the synthesis of 5-HT. In addition to studying the overall effect on 5-HT synthesis, the applicant will determine the effect of sleep deprivation on brain tryptophan concentration, gene expression of tryptophan hydroxylase (TPH, the rate-limiting enzyme in the biosynthetic pathway), and TPH activity. A better understanding of these sleep deprivation-related changes has the potential to advance our knowledge, both in sleep physiology and antidepressant mechanisms of action.

描述(申请者摘要)：这位临床科学家导师 发展奖申请包括一项职业发展和 为申请者Faustino Lopez-Rodriguez，M.D.，Ph.D.提供研究培训 申请者的最终目标是发展成为一名独立的调查员 研究抗抑郁药作用机制的神经生物学。职业生涯 发展和研究计划旨在使申请者能够 这个目标。申请人打算在以下方面补充其背景 电生理学与神经化学技术(生物来源的微透析 行为动物中的单胺和高效液相色谱)和 分子生物学，他计划在获奖期间获得这一学位。 申请者打算应用他在睡眠生理学方面的专业知识来学习 抗抑郁作用的神经生物学机制 睡眠不足。在这项提案中，作为整体研究的第一步 计划，申请者将集中在睡眠剥夺的影响 5-羟色胺(5-HT)活性。他的具体目标如下。1)睡眠 剥夺诱导前脑结构和IS中5-羟色胺释放的变化 并伴有中缝持续高水平的5-羟色胺。2)睡眠不足 改变5-HT1A和5-HT1B自身受体的活性。申请人 假设睡眠剥夺可阻断5-HT1a和/或5HT1b的作用 5-羟色胺释放的激动剂以及5-HT1a和5-HT1a的合成 5-HT1B自身受体。3)睡眠剥夺影响5-羟色胺的合成。在……里面 除了研究对5-羟色胺合成的整体影响外，申请人还将 确定睡眠不足对大脑色氨酸浓度的影响， 植物体内限速酶--色氨酸羟化酶的基因表达 生物合成途径)和TPH活性。更好地理解这些睡眠 与剥夺相关的变化有可能促进我们的知识，无论是在 睡眠生理学和抗抑郁作用机制。

项目成果

Strategic Mental Health Planning and its Practice in China: Retrospect and Prospect.

战略性心理健康规划及其在中国的实践：回顾与展望。

• DOI: 10.11919/j.issn.1002-0829.217025
• 发表时间: 2017
• 期刊: Shanghai archives of psychiatry
• 作者: Xie,Bin

Baseline correction in a two-way randomized blocks design.

双向随机区组设计中的基线校正。

• DOI: 10.1080/10543409208835040
• 发表时间: 1992
• 期刊: Journal of biopharmaceutical statistics
• 影响因子: 1.1
• 作者: Overall,JE;Magee,KN
• 通讯作者: Magee,KN

Methylome and transcriptome signature of bronchoalveolar cells from multiple sclerosis patients in relation to smoking.

• DOI: 10.1177/1352458520943768
• 发表时间: 2021-06
• 期刊: Multiple sclerosis (Houndmills, Basingstoke, England)
• 作者: Ringh MV;Hagemann-Jensen M;Needhamsen M;Kullberg S;Wahlström J;Grunewald J;Brynedal B;Jagodic M;Ekström TJ;Öckinger J;Kular L
• 通讯作者: Kular L