DEVELOPMENT OF AN SPF BABOON COLONY

描述(申请人提供)：这种狒狒长期以来一直被用于 生物医学研究和某些应用具有相当大的优势 而不是其他灵长类物种。尽管它们在研究中被广泛使用，但在那里 目前可供使用的狒狒严重短缺。UOHSC已经 美国第三大狒狒繁殖地，致力于 完善和扩大这一国家资源。一种新的畜牧育种 设施刚刚竣工，可以将现有殖民地扩大到 大约是目前大小的两倍。 就像猕猴所理解的那样，构成正常菌群的病毒 研究动物可以对研究结果和他们的 有效性。众所周知，狒狒体内有许多疱疹病毒的类似物。 以及已知可感染人类和其他灵长类动物的逆转录病毒。尽管有很多人 多年来由不同机构成功繁殖的狒狒，没有蜂群 即使只有一种特定的病毒也不存在。该计划 在这里提出的将导致一个自我维持的殖民地的开始 无所有已知的疱疹病毒、四种逆转录病毒和SV40病毒的狒狒。 为了实现这一目标，研究人员将建立血清学和聚合酶链式反应 检测11种目标病毒中的每一种。这些狒狒病毒将包括 六种疱疹病毒[人类单纯疱疹病毒(HSV)的类似物，水痘 病毒(VZV)、巨细胞病毒(CMV)、人类疱疹病毒6(HHV6)、 Epstein-Barr病毒(EBV)和人类疱疹病毒8型(HHV-8))，四种逆转录病毒 (猿猴泡沫病毒(SFV)、猴D型逆转录病毒(SRV/D)、猿猴 免疫缺陷病毒(SIV)和猴T细胞嗜淋巴病毒(STLV)]和 猴病毒40(SV40)。调查人员将招募不少于12名 在最初的两年里，每年都会有婴儿进入SPF计划，以及 在该计划的3-5年中每年14次，最终总计至少为66 SPF 这些是狒狒。利用计时妊娠，在1-2周内，两名婴儿 将在分娩后12小时内从水坝中移走，并人工饲养 六个月大。所有婴儿都将接受重复测试，以检测每一个 瞄准病毒。在一岁的时候，四到六个SPF的较大的社会群体 动物将会形成。从两到三岁开始，SPF动物 将被整合到更大的社会兼容群体中。这些团体将 最终成熟为SPF动物的小型繁殖母羊。 这一方法将：1)为婴儿提供年龄匹配的伴侣 在早期发展阶段的社会化；2)最小化 从成人向婴儿传播内源性病毒的机会 动物；以及3)允许同时消除许多不同的病毒 来自SPF动物。

DESCRIPTION (Provided by applicant): The baboon has long been used in biomedical research and for certain applications has considerable advantages over other primate species. Despite their widespread use in research, there is currently an acute shortage of baboons available for use. The UOHSC has the third largest baboon breeding colony in the U.S. and is committed to improving and expanding this national resource. A new corral breeding facility has just been completed to allow expansion of the existing colony to approximately twice the current size. As is well appreciated for macaques, viruses constituting the normal flora of research animals can have a considerable effect on research results and their validity. Baboons are known to harbor analogs of many of the herpesviruses and retroviruses known to infect humans and other primates. Despite many years of successful breeding of baboons by various institutions, no colonies of SPF baboons exist that are free of even one specific virus. The program proposed here will result in the beginning of a self-sustaining colony of baboons free of all known herpesviruses, four retroviruses, and SV40. To accomplish this goal, the investigators will establish serological and PCR tests for each of the 11 target viruses. These baboon viruses will include six herpesviruses [analogs of human herpes simplex virus (HSV), varicellazooter virus (VZV), cytomegalovirus (CMV), human herpesviruses 6 (HHV6), Epstein-Barr virus (EBV), and human herpesvirus 8 (HHV-8)), four retroviruses (simian foamy virus (SFV), simian retrovirus type D (SRV/D), simian immunodeficiency virus (SIV), and simian T cell lymphotropic virus (STLV)] and simian virus 40 (SV40). The investigators will recruit no fewer than 12 infant baboons into the SPF program in each of the first two years, and 14/year in years 3-5 of the program for a final total of at least 66 SPF baboons. Utilizing timed pregnancies, over a 1-2 week period, two infants will be removed from their dams within 12 hrs of delivery and hand-reared to six months of age. All infants will be repeatedly tested for each of the target viruses. At one year of age, larger social groups of four to six SPF animals will be formed. Beginning at two to three years of age, SPF animals will be integrated into larger socially compatible groups. These groups will eventually mature into small breeding harems of SPF animals. This approach will: 1) provide infants with an age-matched companion for socialization during their early period of development; 2) minimize opportunities for transmission of endogenous viruses to the infants from adult animals; and 3) allow the simultaneous elimination of many different viruses from SPF animals.