Development of an SPF Baboon Colony

SPF 狒狒群体的发展

• 批准号: 8117086
• 负责人: GARY L WHITE
• 金额: $ 69.99万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-15 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8117086
• 关键词: Age; Animal Experimentation; Animal Model; Animals; Back; Behavioral; Biological Assay; Biomedical Research; Breeding; Communities; Cytomegalovirus; Data; Derivation procedure; Development; Funding; Goals; Growth and Development function; Hand; Herpesviridae; Human; Individual; Infant; Institution; Macaca; Maternal antibody; Measles; Modeling; Monkeypox; N.I.H. Research Support; National Heart, Lung, and Blood Institute; Papio; Primates; Protocols documentation; Recruitment Activity; Research; Research Personnel; Resources; Retroviridae; Simian virus 40; Specific Pathogen Frees; Testing; United States National Institutes of Health; Virus; aged; analog; baboon herpesvirus; behavioral health; experience; germ free condition; improved; male; nonhuman primate; programs

DESCRIPTION (provided by applicant): The baboon has long been used in biomedical research and, for certain applications, has considerable advantages over other primate species. As is well appreciated for macaques, viruses constituting the normal flora of research animals can have a considerable effect on research results and their validity. Baboons are known to harbor analogs of many of the herpesviruses and retroviruses known to infect humans and other primates. Despite many years of successful breeding of baboons by various institutions, the nascent SPF baboon program at OUHSC is the only one of its kind in the world. The overall goal of this program is to develop an active, self-supporting breeding colony of baboons that are free of all five known baboon herpesviruses, four retroviruses, SV40, measles and monkeypox and that are available primarily to support NIH- funded biomedical research programs throughout the US. To accomplish this goal we propose the following specific aims:1. Continue derivation and expansion of the SPF colony. Approximately 25 new infants will be recruited into the SPF program each year. During the requested funding period, initial recruits will reach breeding age and the SPF colony should begin to experience "internal additions" via infants born to SPF animals. 2. Improve the "behavioral health" of infants in the SPF program. Protocols for hand-rearing of infants will be progressively modified with professional guidance to improve the overall behavioral health of baboons in the SPF colony as they grow and mature. 3. Establish multi-aged breeding groups of SPF baboons in a new indoor/outdoor corral facility. When the new facility currently under construction is completed, SPF animals will be moved into it to form several breeding groups consisting of animals of varied ages. 4. Provide SPF baboons to NIH-funded researchers. We are now saving back a limited number of SPF infant males to support the critical needs of the NHLBI/NIH and a few other NIH supported research programs that have critical need for SPF baboons. As the colony begins to mature to breeding status, excess males will be made available to the research community for use in NIH-supported biomedical research programs. Animals culled from the program due to sero-conversion to one virus will also be made available for use in research on projects that can use baboons with the specific virus that triggered the culling of the individual baboon. 5. Establish PCR assays for select viruses to analyze breaks in SPF status due to these viruses. CMV and HVP1 have been responsible for the majority of infants culled from the SPF program. In order to identify infants infected with these viruses before maternal antibodies wane, we will develop PCR tests that can detect infected infants.

描述（由申请人提供）：狒狒长期以来一直用于生物医学研究，在某些应用中，与其他灵长类动物相比具有相当大的优势。正如对猕猴的充分理解，构成研究动物正常植物群的病毒可能对研究结果及其有效性产生相当大的影响。已知狒狒体内有许多疱疹病毒和逆转录病毒的类似物，这些病毒会感染人类和其他灵长类动物。尽管许多机构多年来成功地繁殖了狒狒，但OUHSC新生的SPF狒狒计划是世界上唯一的一个。该计划的总体目标是开发一个活跃的、自我支持的狒狒繁殖群，该繁殖群不含所有五种已知的狒狒疱疹病毒、四种逆转录病毒、SV 40、麻疹和猴痘病毒，并且主要用于支持美国全国NIH资助的生物医学研究计划。为实现这一目标，我们提出以下具体目标：1.继续SPF集落的衍生和扩增。每年大约有25名新生儿将被招募到SPF计划中。在申请资助期间，最初的新成员将达到繁殖年龄，SPF群体应开始通过SPF动物所生的婴儿经历“内部添加”。2.改善SPF计划中婴儿的“行为健康”。将在专业指导下逐步修改婴儿的人工饲养方案，以改善SPF群体中狒狒生长和成熟时的整体行为健康。3.在新的室内/室外畜栏设施中建立SPF狒狒的多年龄繁殖组。当目前正在建设的新设施建成后，SPF动物将被移入其中，形成由不同年龄动物组成的几个繁殖组。4.向NIH资助的研究人员提供SPF狒狒。我们现在正在保存有限数量的SPF雄性婴儿，以支持NHLBI/NIH和其他一些NIH支持的研究项目的关键需求，这些项目对SPF狒狒有关键需求。当殖民地开始成熟到繁殖状态时，多余的雄性将被提供给研究界，用于NIH支持的生物医学研究项目。由于血清转化为一种病毒而从该计划中剔除的动物也将用于研究项目，这些项目可以使用携带特定病毒的狒狒，这些病毒引发了对单个狒狒的剔除。5.建立选择病毒的PCR检测方法，以分析这些病毒导致的SPF状态中断。CMV和HVP 1是SPF计划中淘汰的大多数婴儿的原因。为了在母体抗体减弱之前识别感染这些病毒的婴儿，我们将开发可以检测感染婴儿的PCR测试。