Multistressor impacts of ocean acidification and warming on regeneration and biomineralisation in coastal sea urchins

海洋酸化和变暖对沿海海胆再生和生物矿化的多重压力影响

基本信息

批准号：
2600238
负责人：
金额：
--
依托单位：
University of the Highlands and Islands
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2021
资助国家：
英国
起止时间：
2021 至无数据
项目状态：
未结题

来源：
https://gtr.ukri.org/projects?ref=studentship-2600238
关键词：
Multistressor impacts ocean acidification warming

项目摘要

The studentship will centre on two inter-connected questions: How will sea urchins remineralise and regenerate their tissues in a future multi-stressor environment? And Are the effects of multiple climate-change associated stressors additive, antagonistic, or synergistic? It will utilise the sea urchin regeneration assay to investigate multiple-climatic stressors using experimental conditions of ocean acidification (OA) and warming (Fig 1). The phenotypic assay has been developed to visualise and quantify spine and tube feet regeneration from a single ambulacral section, by photographing regrowth underwater and analysing the images. In addition to the phenotypic assay, molecular and cellular analyses will be included: targeted gene expression, in-situ hybridisation, and histology on regenerating tube feet and spines, with the potential for complementary transcriptomics and proteomics, representing analyses across levels of biological organisation from genes, cells, and organismal physiology. Comparison experiments with different species will investigate interspecific variation in stressor susceptibility, and species from different latitudinal distributions will provide insight into cold-adapted or warmer-adapted mechanisms. Elemental ratio analysis of calcium carbonate structures will allow characterisation of magnesium content and provide further insights into possible skeletal trade-offs under OA and elevated temperature conditions.

这些学生将集中在两个相互联系的问题上：海胆将如何回忆和在未来的多压力环境中再生其组织？多种气候变化相关的应激源的影响添加，拮抗或协同作用？它将利用海胆再生测定法使用海洋酸化（OA）和变暖来研究多气候应激源（图1）。该表型测定已开发出通过拍摄水下再生并分析图像来可视化和量化脊柱和管脚的再生。除表型测定外，还将包括分子和细胞分析：靶向基因表达，原位杂交以及在再生管脚和棘突中的组织学，具有互补的转录组和蛋白质组学的潜力，代表来自基因，细胞，细胞，生物体物理学的生物学组织的分析。与不同物种的比较实验将研究应激源敏感性的种间变化，来自不同纬度分布的物种将提供对冷适应或变暖的机制的见解。碳酸钙结构的元素比分析将允许表征镁含量，并为OA和升高温度条件下的骨骼折衷提供进一步的见解。