MEDIA PREPARATION

媒体准备

• 批准号: 7102514
• 负责人: PETER D. EMANUEL
• 金额: $ 10.24万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-28 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7102514
• 关键词: biomedical facility; cell bank /registry; chemical registry /resource; molecular biology; neoplasm /cancer; reagent /indicator; tissue /cell culture; tissue /cell preparation

DESCRIPTION (provided by applicant): The Media Preparation Shared Facility (MPSF) of the UAB Comprehensive Cancer Center (UAB-CCC) serves as a centralized resource for (a) eukaryotic and bacterial cell culture media and supplements, (b) buffers and other high-volume reagents used in routine cell culture and molecular biology applications, (c) quality control assurance and testing, and (d) general consultation and education with regard to cell culture and molecular biology reagents. The overall goals are to improve economy, efficiency and quality of UAB-CCC research activities. MPSF provides economy by supplying common culture media, supplements and buffers to UAB-CCC faculty at charge-back prices lower than available commercial prices. This is accomplished via (1) on-site production with charge-back rates that fail to generate excess MPSF income, and (2) volume purchase negotiations with commercial suppliers, yielding price and shipping charge reductions not available to the average research laboratory. MPSF offers efficiency by convenient on-site product/service availability, with less paperwork and faster delivery as compared to commercial suppliers. Many users take possession of MPSF products in less than 2 hours after initial request. MPSF promotes quality by (a) performing assays for endotoxin and sterility in on-site products, as well as for endotoxin in investigator-submitted samples, and on-site autoclave performance, and (b) offering personalized services including professional consultation for specific experimental problems at no cost, plus design and preparation of custom reagents at low cost.

描述（由申请人提供）：UAB的媒体准备共享设施（MPSF） 综合性癌症中心（UAB-CCC）是（a）真核生物和细菌细胞培养基和补充剂的集中资源，（b）缓冲液和其他用于常规细胞培养和分子生物学应用中的高容量试剂，（c）质量控制的保证和测试，以及（D）对细胞培养物的一般咨询和教育。 总体目标是提高UAB-CCC研究活动的经济，效率和质量。 MPSF通过向UAB-CCC教职员工供应，以低于可用的商业价格的价格向UAB-CCC教师提供常见的培养基，补充剂和缓冲。 这是通过（1）现场生产来完成的，其抵押税率未能产生多余的MPSF收入，以及（2）与商业供应商进行的数量购买谈判，从而产生了价格和降低运输费用的平均研究实验室。 MPSF通过方便的现场产品/服务可用性提供效率，与商业供应商相比，文书工作和更快的交付速度更少。 最初要求后不到2小时，许多用户在不到2个小时内拥有MPSF产品。 MPSF通过（a）在现场产品中对内毒素和无菌性进行测定，以及在调查员提取的样品中的内毒素以及内毒素的质量，现场高压灭菌性能，以及（b）提供个性化服务，包括无需成本的特定实验问题，以及以低成本的设计和制定定制纪念品的专业咨询。