TRANSGENIC ANIMAL

转基因动物

• 批准号: 7414794
• 负责人: PETER D. EMANUEL
• 金额: $ 20.02万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7414794
• 关键词: Breeding; Comprehensive Cancer Center; Consultations; Cryopreservation; DNA; Development; Disease; Embryo; Embryo Transfer; Fertilization; Fertilization in Vitro; Gene Targeting; Genes; Human; In Vitro; Injection of therapeutic agent; Mammalian Oviducts; Microinjections; Molecular Diagnosis; Mus; Mutation; Production; Reproduction; Reproductive Techniques; Research Personnel; Resource Sharing; Resources; Screening procedure; Services; Superovulation; Training; Transgenes; Transgenic Animals; Transgenic Mice; Transgenic Mouse Facility; Transgenic Organisms; University of Alabama at Birmingham Cancer Center; blastocyst; cancer therapy; cost effective; design; egg; embryonic stem cell; genome sequencing; interest; member; mouse model; pathogen; sperm cell; stem

DESCRIPTION (provided by applicant): The Transgenic Animal/ES Cell Resource Shared Facility is an essential component of the UAB Comprehensive Cancer Center. With completion of the human and mouse genome sequences, genetically modified mouse models are more essential than ever in elucidating disease mechanisms and developing approaches to therapies for cancer. This shared facility provides unique services for the development of genetically modified mouse models and has a long track record of outstanding service. The specific aims include: 1. Provide efficient, cost effective production of transgenic mouse models for Comprehensive Cancer Center investigators by microinjection of gene constructs into fertilized mouse eggs or ES cells into blastocysts; 2. Provide additional services such as cryopreservation of embryos, embryo rederivation to produce pathogen-free mice, and assisted reproduction techniques (e.g., in vitro fertilization [IVF], superovulation, and embryo transfer); 3. Provide expert consultation services to help investigators design the most effective transgene constructs strategies for molecular diagnosis of transgenic mice, and breeding strategies required for perpetuation of transgenic mouse lines. The services provided are not available through any other mechanism except costly commercial alternatives that do not supply the complete set of services provided by this facility.

描述（由申请人提供）：转基因动物/胚胎干细胞资源共享设施是 UAB 综合癌症中心的重要组成部分。 随着人类和小鼠基因组序列的完成，转基因小鼠模型在阐明疾病机制和开发癌症治疗方法方面比以往任何时候都更加重要。 该共享设施为转基因小鼠模型的开发提供独特的服务，并拥有长期的杰出服务记录。 具体目标包括： 1. 提供高效、具有成本效益的转基因小鼠模型生产 综合癌症中心研究人员将基因构建体显微注射到受精小鼠卵中或将 ES 细胞注射到囊胚中； 2. 提供额外服务，例如胚胎冷冻保存、胚胎再衍生以生产无病原体小鼠以及辅助生殖技术（例如体外受精[IVF]、超数排卵和胚胎移植）； 3. 提供专家咨询服务，帮助研究者设计最有效的转基因构建策略，用于转基因小鼠的分子诊断，以及转基因小鼠品系永存所需的育种策略。 除了不提供该设施所提供的全套服务的昂贵的商业替代方案之外，所提供的服务无法通过任何其他机制获得。