Fluorescent Imaging of Unstable Atherosclerotic Plaques in Blood Vessels

开展的研究将包括优化和进一步发展的Diels-Alder反应类之前在小组内开发，提供更高的收率和更大的产品范围。其中一些产品将被用作荧光探针，用于细胞检测疾病标志物，如动脉粥样硬化斑块和癌细胞。目的：研制一种治疗药物，在与动脉粥样硬化斑块等疾病的靶向标志物接触时，能够选择性地释放药物分子和荧光标记物。目标：-进一步优化和开发Diels-Alder反应类，以生产多种不同的芳香支架。-利用已开发的Diels-Alder反应靶向并生产能够选择性释放的荧光探针，以响应选定的疾病标记物。-利用荧光成像分析来确定所开发的荧光探针对其目标响应条件的响应性，并测量药物分子的释放。潜在的应用和好处：- Diels-Alder反应类别的进一步发展，可以为某些合成支架提供更可行的途径。这可能有利于工业更有效地生产潜在的药物化合物。-生产靶向治疗剂可以同时识别和治疗某些疾病，如冠心病和癌症。这将为动脉粥样硬化斑块和癌细胞的定位提供更多的重要信息，同时也可以在单一剂量内治疗这些问题。这可能对社会健康大有裨益，因为这可能是这些疾病的一种非常有效的早期治疗/识别方法。与研究委员会的相关性：该奖学金由工程和物理科学研究委员会（EPSRC）资助。所进行的研究将与EPSRC相关，因为它将促进物理科学领域（化学）的发展，并有可能通过生产更有效的治疗和识别危及生命的疾病的方法来造福社会。二级导师的相关性：Alex Cresswell博士在周环反应领域具有专业知识，可以为研究的潜在工业应用提供流动设备，这使他在实现我的前两个目标方面非常有用。

ontext: The research carried out will consist of the optimization and further development of the classes of Diels-Alder reactions developed within the group previously, providing higher yields and larger scope of products. Some of these products will then be applied as fluorescent probes for cellular sensing for markers of disease such as atherosclerotic plaques and cancerous cells. Aim: To produce a theranostic agent capable of selectively releasing a drug molecule and a fluorescent marker upon contact with targeted markers of disease such as atherosclerotic plaques.Objectives:-Further optimise and develop the Diels-Alder reaction classes previously developed within the group to produce numerous varied aromatic scaffolds. -Target and produce a fluorescent probe capable of selective release in response to chosen disease markers utilising the developed Diels-Alder reactions.-Utilise fluorescent imaging assays to determine the responsiveness of the developed fluorescent probe to its targeted response conditions and measure the release of the drug molecule alongside this.Potential Applications and Benefits: -The further development of the Diels-Alder reaction classes which were previously identified within the group could allow for a more viable route to certain synthetic scaffolds. This could be of benefit within industry to produce potential pharmaceutical compounds more effectively.-The production of targeted theranostic agents could allow for the simultaneous identification and treatment of certain diseases such as coronary heart disease and cancers. This would provide a significant amount more information to do with the location of atherosclerotic plaques and cancerous cells whilst also treating these issues within a singular dose. This could be of great benefit to the health of society as a potentially highly effective early treatment/identification method for these diseases.Relevance to Research Council: This studentship is funded by the Engineering and Physical Sciences Research Council (EPSRC). The research undertaken will be of relevance to the EPSRC as it will yield developments within the physical sciences field (chemistry) with the potential to yield benefit to society through the production of more effective methods of treating and identifying life-threatening diseases.Relevance of Secondary Supervisor: Dr. Alex Cresswell has expertise within the field of pericyclic reactions and can provide flow apparatus for potential industrial applications of the research, making him highly useful in achieving my first two objectives.