Unravelling the molecular basis of fidelity in mRNA splicing

揭示 mRNA 剪接保真度的分子基础

• 批准号: 2603123
• 金额: --
• 依托单位: Institute of Cancer Research
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2603123
• 关键词: Unravelling; molecular; basis; fidelity; mRNA

Splicing of pre-mRNA is a crucial process in gene expression in eukaryotes, linked to human diseases such as cancer. It is catalysed by the spliceosome, a large protein-RNA complex which undergoes major conformational and compositional changes to enable the splicing reactions and release of the spliced mRNA. Structural characterisation by CryoEM and X-ray crystallography have greatly enhanced the understanding of the molecular basis of splicing. However, the processes ensuring proofreading and fidelity mechanisms of the splicing remain structurally poorly understood. This project aims to elucidate these processes by the structural characterization of fidelity complexes identified in our laboratories by cryoEM.

前体mRNA的剪接是真核生物基因表达的关键过程，与人类疾病如癌症有关。它由剪接体催化，剪接体是一种大型蛋白质-RNA复合物，其经历主要的构象和组成变化以使剪接反应和剪接mRNA的释放成为可能。通过CryoEM和X射线晶体学的结构表征极大地增强了对剪接的分子基础的理解。然而，确保剪接的校对和保真度机制的过程在结构上仍然知之甚少。该项目旨在阐明这些过程中的保真度复合物的结构表征，在我们的实验室确定的cryoEM。