ABI 3730 Capillary Sequencers

描述（由申请人提供）：在后人类基因组时代，基因表达的遗传信息和功能测定已成为NIH资助研究的重要组成部分。我们正在申请资金购买两台自动毛细管测序仪，以改善伊利诺伊大学香槟分校的W。M.凯克比较和功能基因组学中心。新的测序仪大大提高了通量、可靠性和性能，并以大大降低的劳动力、耗材和维护成本进行操作。这些成本节约将体现在用户的测序成本降低，NIH资助的实验室预算增加，以及生产更短的周转时间和更高质量的数据。此外，随着操作和维护老化测序仪的成本增加，升级到更高效和更经济的测序仪变得至关重要。随着几个NIH资助的功能基因组学项目在UIUC的资金，在测序服务的持续增加进行自凯克中心成立以来，和校园生物医学研究人员的重大扩张与UIUC后基因组研究所的建立，在我们的测序需求大幅增加正在发生。 凯克中心的高重复测序和基因分型实验室是一个综合基因组学设施的一部分，为研究人员提供全面的，最先进的仪器，咨询和培训机会，并占据了大型测序中心所不提供的独特利基。

DESCRIPTION (provided by applicant): In the post human genomic era, genetic information and functional assays of gene expression have become indispensable for a significant fraction of NIH-funded research. We are requesting funds to purchase two automated capillary sequencers to improve sequencing operations provided by the University of Illinois at Urbana-Champaign's W. M. Keck Center for Comparative and Functional Genomics. New sequencers have greatly improved throughput, reliability, performance, and are operated at greatly reduced costs for labor, consumables, and maintenance. These cost savings will be reflected in lower sequencing costs for users, stretching NIH-funded lab's budgets, and producing shorter turnaround times and higher quality data. Also, as the cost of operating and maintaining aging sequencers increases, upgrading to more efficient and economical sequencers becomes essential. With the funding of several NIH-funded functional genomics projects at the UIUC, the continuous increase in sequencing services performed since the establishment of the Keck Center, and the major expansion of campus biomedical researchers with the establishment of the UIUC Post-Genomic Institute, large increases in our sequencing needs are occurring. The Keck Center's High Throughput Sequencing and Genotyping lab is part of an integrated genomics facility providing comprehensive, state-of-the-art instrumentation, consultation, and training opportunities for researchers, and occupies a unique niche not provided by large sequencing centers.