ABI 3730 Capillary Sequencers

描述(由申请人提供)：在后人类基因组时代，遗传信息和基因表达的功能分析已经成为NIH资助的研究的重要组成部分。我们正在申请资金购买两台自动毛细管测序仪，以改善伊利诺伊大学香槟分校W.M.Keck比较和功能基因组学中心提供的测序操作。新的测序仪极大地提高了吞吐量、可靠性和性能，并且大大降低了人工、耗材和维护成本。这些成本节约将体现在为用户降低测序成本、延长NIH资助的实验室预算、产生更短的周转时间和更高质量的数据上。此外，随着操作和维护老化测序仪的成本增加，升级到更高效和更经济的测序仪变得至关重要。随着美国国立卫生研究院资助的几个UIUC功能基因组学项目的资助，自凯克中心成立以来提供的测序服务不断增加，以及UIUC后基因组研究所的建立，校园生物医学研究人员的重大扩张，我们的测序需求正在大幅增加。 凯克中心的高通量测序和基因分型实验室是综合基因组学设施的一部分，为研究人员提供全面、最先进的仪器、咨询和培训机会，并占据大型测序中心所不具备的独特利基市场。

DESCRIPTION (provided by applicant): In the post human genomic era, genetic information and functional assays of gene expression have become indispensable for a significant fraction of NIH-funded research. We are requesting funds to purchase two automated capillary sequencers to improve sequencing operations provided by the University of Illinois at Urbana-Champaign's W. M. Keck Center for Comparative and Functional Genomics. New sequencers have greatly improved throughput, reliability, performance, and are operated at greatly reduced costs for labor, consumables, and maintenance. These cost savings will be reflected in lower sequencing costs for users, stretching NIH-funded lab's budgets, and producing shorter turnaround times and higher quality data. Also, as the cost of operating and maintaining aging sequencers increases, upgrading to more efficient and economical sequencers becomes essential. With the funding of several NIH-funded functional genomics projects at the UIUC, the continuous increase in sequencing services performed since the establishment of the Keck Center, and the major expansion of campus biomedical researchers with the establishment of the UIUC Post-Genomic Institute, large increases in our sequencing needs are occurring. The Keck Center's High Throughput Sequencing and Genotyping lab is part of an integrated genomics facility providing comprehensive, state-of-the-art instrumentation, consultation, and training opportunities for researchers, and occupies a unique niche not provided by large sequencing centers.