Protein Identification Core

蛋白质鉴定核心

• 批准号: 7620687
• 负责人: Jonathan V. Sweedler
• 金额: $ 41.36万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2014-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7620687
• 关键词: Address; Arts; Bioinformatics; Biological; Brain; Cells; Communities; Complement; Complex; Custom; Data; Development; Equus caballus; Fingerprint; Fourier Transform; Fourier transform ion cyclotron resonance; Goals; Heart; Image; Imaging Techniques; Investigation; Laboratories; Mass Spectrum Analysis; Measurement; Methods; Mind; Modeling; National Institute of Drug Abuse; Nervous system structure; Neurobiology; Neurons; Organism; Peptide Signal Sequences; Peptides; Post-Translational Protein Processing; Protein Analysis; Proteins; Proteomics; Reading; Research; Sample Size; Sampling; Signal Transduction; Signaling Molecule; Structure; Techniques; Tissues; Work; base; gel electrophoresis; improved; instrument; instrumentation; intercellular communication

The overall goal of the Protein Identification Core of the UIUC Neuroproteomics Center on Cell-Cell Signaling is to characterize the protein and peptide complements of a range of samples, with a special emphasis on those involved in intercellular signaling. The intracellular signaling molecules expressed in the cells and tissues under investigation present an extremely complex analytical challenge with a vast number of components, varying dramatically in size and concentration. To address these challenges, and to provide our users with a battery of state-of-the-art protein identification techniques, a range of mass spectrometric approaches are used, including differential gel electrophoresis, mass fingerprinting, tandem sequencing, accurate mass measurements, and top-down intact protein analysis. In addition, spatial localization of intercellular signaling molecules is performed using a variety of mass spectrometric imaging techniques. Our user's needs for intercellular signaling molecule identification and quantitation in the brain are quite different from what is required from proteomics measurements of unicellular organisms and homogenous tissues, particularly with regard to sample size and complexity. Thus, the advanced measurement strategies used in our Center reflect this complexity. With the combined expertise and facilities in the research groups associated with this core, a unique opportunity exists to identify and characterize intercellular signaling peptides and proteins across a surprising range of models and scales.

UIUC神经蛋白质组学中心细胞-细胞蛋白质鉴定核心的总体目标 信号是用来表征一系列样品的蛋白质和肽的补体，具有 特别强调那些参与细胞间信号传递的人。细胞内信号分子 在研究中的细胞和组织中的表达呈现出极其复杂的分析 具有大量组件的挑战，这些组件在大小和集中度上都有很大不同。至 应对这些挑战，并为我们的用户提供最先进的蛋白质电池 鉴定技术，使用了一系列的质谱学方法，包括差示 凝胶电泳、质量指纹、串联测序、准确的质量测量，以及 自上而下的完整蛋白质分析。此外，细胞间信号分子的空间定位 使用各种质谱学成像技术进行。我们的用户对 大脑中细胞间信号分子的识别和定量与 单细胞生物和同质组织的蛋白质组学测量需要什么， 特别是在样本大小和复杂性方面。因此，先进的测量策略 在我们中心的使用反映了这种复杂性。与研究中的专业知识和设施相结合 与这一核心相关的群体，存在着识别和表征的独特机会 细胞间信号肽和蛋白质跨越一系列令人惊讶的模型和规模。