Protein Identification Core

UIUC神经蛋白质组学中心细胞-细胞蛋白质鉴定核心的总体目标 信号转导是为了表征一系列样品的蛋白质和肽互补物，具有 特别强调那些参与细胞间信号传导的。细胞内信号分子 在所研究的细胞和组织中表达呈现出极其复杂的分析 挑战涉及大量组件，其尺寸和浓度差异很大。至 解决这些挑战，并为我们的用户提供最先进的蛋白质电池 鉴定技术，使用了一系列质谱方法，包括微分法 凝胶电泳、质量指纹、串联测序、精确质量测量，以及 自上而下的完整蛋白质分析。此外，细胞间信号分子的空间定位 使用多种质谱成像技术进行。我们用户的需求 大脑中细胞间信号分子的识别和定量与大脑中的细胞间信号分子有很大不同 单细胞生物和同质组织的蛋白质组学测量需要什么， 特别是在样本量和复杂性方面。因此，先进的测量策略 我们中心的使用反映了这种复杂性。凭借研究领域的综合专业知识和设施 与此核心相关的群体，存在独特的机会来识别和表征 细胞间信号肽和蛋白质的模型和规模令人惊讶。

The overall goal of the Protein Identification Core of the UIUC Neuroproteomics Center on Cell-Cell Signaling is to characterize the protein and peptide complements of a range of samples, with a special emphasis on those involved in intercellular signaling. The intracellular signaling molecules expressed in the cells and tissues under investigation present an extremely complex analytical challenge with a vast number of components, varying dramatically in size and concentration. To address these challenges, and to provide our users with a battery of state-of-the-art protein identification techniques, a range of mass spectrometric approaches are used, including differential gel electrophoresis, mass fingerprinting, tandem sequencing, accurate mass measurements, and top-down intact protein analysis. In addition, spatial localization of intercellular signaling molecules is performed using a variety of mass spectrometric imaging techniques. Our user's needs for intercellular signaling molecule identification and quantitation in the brain are quite different from what is required from proteomics measurements of unicellular organisms and homogenous tissues, particularly with regard to sample size and complexity. Thus, the advanced measurement strategies used in our Center reflect this complexity. With the combined expertise and facilities in the research groups associated with this core, a unique opportunity exists to identify and characterize intercellular signaling peptides and proteins across a surprising range of models and scales.