Sampling and Separation Core

采样和分离核心

• 批准号: 7620681
• 负责人: Jonathan V. Sweedler
• 金额: $ 24.17万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2014-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7620681
• 关键词: Area; Bioinformatics; Biological; Biological Assay; Brain; Brain region; Cell Nucleus; Cells; Chemicals; Chemistry; Complement; Ensure; Equipment; Experimental Designs; Fractionation; Goals; Heterogeneity; Image; Investigation; Link; Liquid substance; Mass Spectrum Analysis; Measurement; Measures; Methodology; Methods; Microdialysis; Nervous system structure; Neurobiology; Neurons; Neurosciences; Peptide Signal Sequences; Peptides; Pharmaceutical Preparations; Preparation; Process; Proteins; Proteomics; Protocols documentation; Sampling; Signaling Molecule; Spatial Distribution; System; Techniques; Technology; Tissues; Work; base; brain tissue; flexibility; intercellular communication; interest; research study; success

The overarching goal of the Sampling and Separation Core is to support diverse measurements of signaling peptides and proteins using a range of approaches and then provide these samples to the Protein Identification Core for analysis. The chemical, temporal and spatial diversity of cell-cell signaling molecules requires significant expertise and planning to allow successful peptidomics and proteomics measurements. To profile signaling peptides and proteins in specific brain tissues and cells, this core specializes in: (1) collecting and fractionating peptides and proteins from biological samples, (2) creating directed sampling methodologies for single cells, spatially distinct brain regions, and biological fluids, (3) optimizing sample fractionation approaches including low-volume multidimensional separations, and (4) preparing tissues for mass spectrometry imaging. In cases where protocols and techniques do not exist for to accomplish the measurements goals, the appropriate technologies are developed. This suite of technologies will be applied to profile the signaling peptides and proteins within the nervous system in the study of fundamental neuroscience as well as pathological conditions such as drug-of-abuse-induced changes. Samples range from intact brain regions to single cells, from probing the chemical content of a tissue to measuring the subset that undergoes activity-dependent release, and from acquiring information on spatial to temporal changes in the signaling peptide complement. The core offers the flexibility to individually tailor isolation and separation methodologies for signaling peptides and related proteins inherently unique to the neurobiological phenomena under investigation by each major user.

采样和分离核心的首要目标是支持对以下各项的不同测量： 信号肽和蛋白质，然后将这些样品提供给 用于分析的蛋白质鉴定核心。细胞间的化学、时间和空间多样性 信号分子需要大量的专业知识和计划，以允许成功的肽组学和 蛋白质组学测量。分析特定脑组织中的信号肽和蛋白质， 细胞，这个核心专门从事：（1）收集和分级肽和蛋白质从生物 样本，（2）创建单细胞，空间上不同的大脑定向采样方法 区域和生物流体，（3）优化样品分级方法，包括低体积 多维分离，和（4）制备用于质谱成像的组织。情况下 在不存在用于实现测量目标的协议和技术的情况下， 开发适当的技术。这套技术将用于分析 基础神经科学研究中的神经系统内的信号肽和蛋白质 以及病理状况如滥用药物引起的变化。样品范围从 完整的大脑区域到单个细胞，从探测组织的化学成分到测量细胞的化学成分， 经历活动依赖性释放的子集，以及从获取空间信息到 信号肽补体的时间变化。核心提供了灵活性， 用于信号肽和相关蛋白质定制分离和分离方法 每个主要用户所研究的神经生物学现象都是独一无二的。