Sampling and Separation Core

采样和分离核心的总体目标是支持以下各项测量 使用一系列方法传递多肽和蛋白质信号，然后将这些样本提供给 蛋白质鉴定核心用于分析。细胞间的化学、时间和空间多样性 信号分子需要丰富的专业知识和规划，才能成功地进行多肽和 蛋白质组学测量。对特定脑组织中的信号肽和蛋白质进行分析 细胞，这个核心专门从事：(1)从生物中收集和分离多肽和蛋白质 样本，(2)创建单个细胞的定向采样方法，空间上不同的大脑 (3)优化样品分离方法，包括低体积 多维分离，以及(4)为质谱学成像准备组织。在案件中 如果不存在用于实现测量目标的协议和技术， 开发了适当的技术。这套技术将被应用于分析 基础神经科学研究中神经系统内的信号肽和蛋白质 以及病理条件，如滥用药物引起的变化。样品的范围从 从完整的脑区到单个细胞，从探测组织的化学成分到测量 经历依赖于活动的释放的子集，并从获取空间信息到 信号肽补体的时间变化。核心提供了灵活性，可以单独 为信号肽和相关蛋白量身定做的分离和分离方法 这是每个主要使用者正在研究的神经生物学现象所独有的。

The overarching goal of the Sampling and Separation Core is to support diverse measurements of signaling peptides and proteins using a range of approaches and then provide these samples to the Protein Identification Core for analysis. The chemical, temporal and spatial diversity of cell-cell signaling molecules requires significant expertise and planning to allow successful peptidomics and proteomics measurements. To profile signaling peptides and proteins in specific brain tissues and cells, this core specializes in: (1) collecting and fractionating peptides and proteins from biological samples, (2) creating directed sampling methodologies for single cells, spatially distinct brain regions, and biological fluids, (3) optimizing sample fractionation approaches including low-volume multidimensional separations, and (4) preparing tissues for mass spectrometry imaging. In cases where protocols and techniques do not exist for to accomplish the measurements goals, the appropriate technologies are developed. This suite of technologies will be applied to profile the signaling peptides and proteins within the nervous system in the study of fundamental neuroscience as well as pathological conditions such as drug-of-abuse-induced changes. Samples range from intact brain regions to single cells, from probing the chemical content of a tissue to measuring the subset that undergoes activity-dependent release, and from acquiring information on spatial to temporal changes in the signaling peptide complement. The core offers the flexibility to individually tailor isolation and separation methodologies for signaling peptides and related proteins inherently unique to the neurobiological phenomena under investigation by each major user.