ABI PRISM 7900HT Sequence Detection System

描述（由申请人提供）：随着最近实验室仪器的快速爆炸，遗传学已成为一个技术驱动的领域。 然而，高科技伴随着高价格标签。事实证明，除了规模最大、资金最充足的实验室之外，任何实验室都不可能找到资源，随着研究的进展，从一种尖端技术转移到另一种尖端技术。为了解决这个问题，加州大学洛杉矶分校承诺通过提供最先进的核心设施系统来支持和促进所有科学家的研究。这些核心的人员配备和设备，使科学家能够将更多的时间集中在解决智力问题上，而不是获取和学习新技术。加州大学洛杉矶分校的基因测序和基因分型核心设施，其咨询委员会由世界上一些顶尖的遗传学家组成，并与当地科学界的一个非常庞大的用户群建立了密切的关系，不仅能够确定遗传研究的未来方向，而且能够确定当地科学家的需求。我们已经确定了一个廉价的方法来产生大量的SNP基因型，并进行基因表达实验，一旦基因已被确定的技术的强烈需求。测序和基因分型核心目前提供了几种SNP基因分型技术，并操作ABI 7700 Taqman仪器进行基因表达实验。利用Taqman技术，SNP基因分型和基因表达都可以在一个平台上进行。然而，核心目前维护的仪器在每个反应的成本和吞吐量方面都不足以跟上这两种应用研究的不断发展的要求。高通量ABI 7900HT Taqman平台将作为一种解决方案，采用Taqman技术在一台价格适中的仪器上提供大规模SNP基因分型、定量PCR和基因表达功能。 测序和基因分型核心已经在核心目前的ABI 7700上拥有丰富的技术经验，沿着经过验证的基础设施和专业知识来维护和管理该技术，无论是在实验室的上游还是在数据管理和分析领域的下游。ABI 7900HT可以迅速上线，并为加州大学洛杉矶分校和更广泛的科学界的NIH资助的科学家的大量现有和不断扩大的客户群提供有价值的服务。

DESCRIPTION (provided by applicant): With the recent rapid explosion in laboratory instrumentation, genetics has become a technology-driven field. High technology, however, comes with a high price tag. It can prove impossible for any but the largest, most well-funded laboratories to find the resources to move from one cutting-edge technology to the next as their research progresses. To address this problem, the University of California, Los Angeles has made a commitment to supporting and facilitating the research of all its scientists by providing a system of state-of-the-art Core facilities. These Cores are staffed and equipped to allow scientists to concentrate more time on intellectual problem solving, and less on acquiring and learning new technologies. The UCLA Sequencing and Genotyping Core Facility, with its Advisory Committee made up of some of the top geneticists in the world, and its close relationship with a very large user base in the local scientific community, is in an excellent position to identify not only the future direction of genetic research, but also the needs of local scientists. We have identified a strong need for an inexpensive method to generate large numbers of SNP genotypes, and a technology to perform gene expression experiments once the genes have been identified. The Sequencing and Genotyping Core currently offers several technologies for SNP genotyping, and also operates an ABI 7700 Taqman instrument for gene expression experiments. With Taqman technology, both SNP genotyping and gene expression are possible on a single platform. However, the instruments the Core currently maintains are inadequate in cost per reaction and in throughput to keep up with the evolving requirements of research with these two applications. The high-throughput ABI 7900HT Taqman platform would serve as a solution, employing Taqman technology to offer large-scale SNP genotyping, quantitative PCR, and gene expression capabilities all on a single, moderately priced instrument. The Sequencing and Genotyping Core already has extensive experience with this technology on the Core's current ABI 7700, along with the proven infrastructure and expertise to maintain and administer the technology, both upstream in the laboratory and downstream in the data management and analysis arenas. The ABI 7900HT could be rapidly brought on-line, and provide a valuable service to a large existing and expanding client base of NIH-funded scientists at UCLA and in the broader scientific community.