ABI PRISM 7900HT Sequence Detection System

描述（申请人提供）：随着近年来实验室仪器的快速发展，遗传学已经成为一个技术驱动的领域。然而，高科技伴随着高昂的价格标签。事实可能证明，除了规模最大、资金最充足的实验室之外，任何实验室都不可能随着研究的进展，找到从一种尖端技术转移到另一种尖端技术的资源。为了解决这个问题，加州大学洛杉矶分校承诺通过提供最先进的核心设施系统来支持和促进所有科学家的研究。这些中心配备了人员和设备，使科学家能够把更多的时间集中在解决智力问题上，而不是在获取和学习新技术上。加州大学洛杉矶分校测序和基因分型核心设施的咨询委员会由世界上一些顶尖的遗传学家组成，它与当地科学界的庞大用户群有着密切的关系，不仅可以确定基因研究的未来方向，还可以确定当地科学家的需求。我们已经确定了对一种廉价的方法产生大量SNP基因型的强烈需求，以及一种一旦基因被确定就进行基因表达实验的技术。测序和基因分型中心目前提供多种SNP基因分型技术，并操作ABI 7700 Taqman基因表达实验仪器。有了Taqman技术，SNP基因分型和基因表达都可以在一个平台上实现。然而，核心目前维持的仪器在每个反应的成本和吞吐量方面都不足，无法跟上这两种应用的不断变化的研究需求。高通量ABI 7900HT Taqman平台将作为解决方案，采用Taqman技术，在一个价格适中的仪器上提供大规模SNP基因分型，定量PCR和基因表达功能。测序和基因分型核心已经在核心当前的ABI 7700上拥有丰富的技术经验，以及在实验室上游和数据管理和分析领域下游维护和管理该技术的成熟基础设施和专业知识。ABI 7900HT可以迅速上线，并为加州大学洛杉矶分校的nih资助的科学家和更广泛的科学界提供有价值的服务。

DESCRIPTION (provided by applicant): With the recent rapid explosion in laboratory instrumentation, genetics has become a technology-driven field. High technology, however, comes with a high price tag. It can prove impossible for any but the largest, most well-funded laboratories to find the resources to move from one cutting-edge technology to the next as their research progresses. To address this problem, the University of California, Los Angeles has made a commitment to supporting and facilitating the research of all its scientists by providing a system of state-of-the-art Core facilities. These Cores are staffed and equipped to allow scientists to concentrate more time on intellectual problem solving, and less on acquiring and learning new technologies. The UCLA Sequencing and Genotyping Core Facility, with its Advisory Committee made up of some of the top geneticists in the world, and its close relationship with a very large user base in the local scientific community, is in an excellent position to identify not only the future direction of genetic research, but also the needs of local scientists. We have identified a strong need for an inexpensive method to generate large numbers of SNP genotypes, and a technology to perform gene expression experiments once the genes have been identified. The Sequencing and Genotyping Core currently offers several technologies for SNP genotyping, and also operates an ABI 7700 Taqman instrument for gene expression experiments. With Taqman technology, both SNP genotyping and gene expression are possible on a single platform. However, the instruments the Core currently maintains are inadequate in cost per reaction and in throughput to keep up with the evolving requirements of research with these two applications. The high-throughput ABI 7900HT Taqman platform would serve as a solution, employing Taqman technology to offer large-scale SNP genotyping, quantitative PCR, and gene expression capabilities all on a single, moderately priced instrument. The Sequencing and Genotyping Core already has extensive experience with this technology on the Core's current ABI 7700, along with the proven infrastructure and expertise to maintain and administer the technology, both upstream in the laboratory and downstream in the data management and analysis arenas. The ABI 7900HT could be rapidly brought on-line, and provide a valuable service to a large existing and expanding client base of NIH-funded scientists at UCLA and in the broader scientific community.