AB 3730XL, 96 CAP DNA ANALYZER

该子项目是利用由NIH/NCRR资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：对基因分型的需求正在迅速增加，通过大型关联研究对复杂性状的研究，有大量的样本和snp。与此同时，对微卫星基因分型和测序的需求至少以目前的水平继续存在。因此，快速、廉价、高通量测序和基因分型的能力必须提高。近年来实验室仪器的快速发展完全能够满足这一日益增长的需求。然而，这种高科技的代价也很高。事实可能证明，除了规模最大、资金最充足的实验室之外，任何实验室都不可能随着研究的进展，找到从一种尖端技术转移到另一种尖端技术的资源。为了解决这个问题，加州大学洛杉矶分校承诺通过提供最先进的核心设施系统来支持和促进所有科学家的研究。这些中心配备了人员和设备，使科学家能够把更多的时间集中在解决智力问题上，而不是在获取和学习新技术上。加州大学洛杉矶分校测序和基因分型核心服务于当地科学界的大量用户群的研究需求。我们已经确定需要一种廉价的方法来产生越来越多的SNP基因型。为了满足这一需求，同时继续支持我们所服务的科学家的测序和微卫星需求，核心需要更新其能力。我们正在申请资金购买AB 3730XL， 96毛细管DNA分析仪。核心已经成功地操作了这种技术的旧型号超过五年。目前有AB 3700毛细管仪两台，AB 3730S一台，48台毛细管仪。这三种仪器目前已接近满负荷运转，每天都能产生DNA序列、微卫星和snp。这3700台仪器已经连续运行了四年，很快就需要更换。3700不能运行SNP基因型，它产生的序列具有更短的读取长度和不如3730的质量。3700的效率较低，更容易发生故障，并且比3730的运行成本和每个样本成本更高。该模型已停止使用，在所要求的供资期限的两年内将不会得到支助。因此，有必要用所要求的3730仪器替换3700。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The demand for genotyping is increasing rapidly, fuelled by research into complex traits through large association studies, with large numbers of samples and SNPs. At the same time, the need for microsatellite genotyping and sequencing continues to exist in at least their current levels. Therefore, the capacity for rapid, cheap, high-throughput sequencing and genotyping must increase. The recent rapid explosion in laboratory instrumentation is well able to keep pace with this increasing need. However, this high technology comes with a high price tag. It can prove impossible for any but the largest, most well-funded laboratories to find the resources to move from one cutting-edge technology to the next as their research progresses. To address this problem, the University of California, Los Angeles has made a commitment to supporting and facilitating the research of all its scientists by providing a system of state-of-the-art Core facilities. These Cores are staffed and equipped to allow scientists to concentrate more time on intellectual problem solving, and less on acquiring and learning new technologies. The UCLA Sequencing and Genotyping Core serves the research needs of a large user base in the local scientific community. We have identified a coming need for an inexpensive method to generate increasingly large numbers of SNP genotypes. In order to meet this need, while at the same time continuing to support the sequencing and microsatellite requirements of the scientists we serve, the Core needs to update its capacity. We are requesting funds for an AB 3730XL, 96 Capillary DNA Analyzer. The Core has been successfully operating older models of this technology for over five years. Currently there are two AB 3700 capillary instruments, and one AB 3730S, 48 capillary instrument. These three instruments are currently running close to capacity, generating DNA sequence, microsatellites, and SNPs on a daily basis. The 3700 instruments have been in continuous operation for four years, and will need to be replaced very soon. The 3700 cannot run SNP genotypes, and the sequence it produces has shorter read lengths and inferior quality to the 3730. The 3700 is less efficient, more prone to break-down, and has higher running costs and per-sample costs than the 3730. The model has been discontinued, and will not be supported within two years of the requested funding period. Therefore, it is necessary to replace the 3700s with the requested 3730 instrument.