BioMark MX/HX Genetic Analysis System

描述（由申请人提供）：下一代测序在基因组学工具包中增加了一个非常宝贵的新工具，并且正在引起基因组研究设计的范式转变，这一点几乎没有争议。下一代测序技术使人们能够更深入地研究基因组的结构和功能，使这项技术广泛地应用于科学界势在必行。为此，加州大学洛杉矶分校目前通过人类遗传学核心实验室向研究界提供四种下一代测序技术：两种Illumina基因组分析仪（Solexas），一种Applied Biosystems SOLiD和一种Roche GS-FLX（454）。加州大学洛杉矶分校有许多正在进行的研究项目使用这项技术，以及大量从事生物医学研究的科学家，这项技术将在不久的将来受益。然而，样品的预处理已经成为一个瓶颈。DNA文库制备是在基因组测序仪上分析样品之前的关键且耗时的步骤。申请的BioMark MX/HX基因分析系统具有用于Illumina、SOLiD和Roche测序仪的文库制备试剂盒。这些试剂盒极大地提高了文库制备的准确性和速度，从而提高了下游数据质量。BioMark系统还使用了其他可用方法的一小部分DNA起始材料，节省了宝贵的DNA样品，并允许分析以前太小而无法分析的样品。快速分析极少量DNA的能力将是本提案中的两个项目的一个特别优势，这两个项目是快速流感监测和研究中心的一部分，该中心调查禽流感的分布和传播风险，这是一个关键的公共卫生问题，需要快速，高通量测序。BioMark系统上可能的第二个关键检测是高通量单细胞基因表达。该检测对于一个主要用户对人类胎儿生殖细胞发育的研究至关重要，并且对于来自加州大学洛杉矶分校的广泛干细胞研究中心（BSCRS）和琼森综合癌症中心（JCCC）的许多核心用户都很有价值。目前校园内没有仪器可以进行BioMark上可能的单细胞检测类型。该系统将为加州大学洛杉矶分校的研究界做出宝贵的贡献，提供将加州大学洛杉矶分校的生物医学研究带入下一个基因组发现时代所需的高通量基因组技术。

DESCRIPTION (provided by applicant): There is little debate that Next Generation Sequencing has added an invaluable new tool in the genomics toolkit, and is causing a paradigm shift in the design of genomic studies. Next Generation Sequencing is enabling a deeper examination into the structure and function of the genome, and it is imperative to make this technology widely available to the scientific community. To this end, UCLA currently offers four Next Generation Sequencing technologies to the research community through the Human Genetics Core laboratories: two Illumina Genome Analyzers (Solexas), one Applied Biosystems SOLiD, and one Roche GS- FLX (454). There are many ongoing research projects at UCLA using this technology, and a large community of scientists engaged in biomedical research for whom this technology will be beneficial in the very near future. However, preprocessing of samples has become a bottleneck. DNA library preparation is a critical and time- consuming step before samples can be analyzed on the genome sequencers. The requested BioMark MX/HX Genetic Analysis System has library preparation kits for the Illumina, SOLiD, and Roche sequencers. These kits offer great improvements in accuracy and speed of library preparation, which in turn improves downstream data quality. The BioMark System also uses a fraction of the amount of DNA starting material as other available methods, saving precious DNA samples and allowing analysis of samples previously too small to analyze. The ability to rapidly analyze very small amounts of DNA will be a particular advantage to the two projects in this proposal that are part of the Center for Rapid Influenza Surveillance and Research which investigates the distribution and transmission risks of avian influenza, a critical public health issue requiring rapid, high-throughput sequencing. A second key assay possible on the BioMark System is high-throughput single cell gene expression. This assay is critical to one major user's research into human fetal germ cell development, and will be valuable to many of the Core's users from UCLA's Broad Stem Cell Research Center (BSCRS) and Jonsson Comprehensive Cancer Center (JCCC). There is currently no instrument on campus that can perform the type of single cell assay possible on the BioMark. This System will make a valuable contribution to the UCLA research community, offering the types of high-throughput genomic technologies necessary to move biomedical research at UCLA into the next era of genomic discovery.