Acquiring Mass Spectrometry for the Midwest Proteome Ctr

描述（由申请人提供）：质谱（MS）是复杂蛋白质样品分析的首选方法。本方案要求使用ABI QSTAR XL混合质谱仪。这款大大改进的仪器包含基质辅助激光解吸/电离飞行时间（MALDI-TOF）和电喷雾电离串联质谱仪（ESI-MSn）的能力，以协助芬奇健康科学大学/芝加哥医学院（FUHS/CMS）的研究人员在蛋白质鉴定/表征和蛋白质组学/功能基因组学领域。目前，FUHS/CMS没有MS能力来满足日益增长的复杂蛋白质样品分析的迫切需求。购买混合质谱仪是在建立“质谱核心设施”的同时提出的，该设施是中西部蛋白质组中心的一部分，是芝加哥/密尔沃基地区的独特基础设施。马克·j·格鲁克斯曼（Marc J. Glucksman）博士最近被招募到CMS，以建立一个蛋白质组学中心，他将领导MS设施。如申请中所述，来自校园6个不同部门的14名研究人员，由美国国立卫生研究院7个不同研究所资助，对这种设备有特殊需求。事实上，由于校园里缺乏仪器，一些研究人员目前被迫与其他机构/公司合作或签订合同来追求他们的研究目标，这种情况经常导致长时间的延迟，低分辨率的数据和阻碍生产力。因此，购买QSTAR XL质谱仪，再加上招聘更多的教师来建立核心设施，将对CMS的生物医学研究产生重大影响。详细的组织计划描述了有效的管理和使用仪器。该大学承诺提供五年的费用分摊支持，包括设置，安装，设备维护，支持人员和设施管理人员的费用。首席研究员吸纳了世界各地的专家，作为该设施外部咨询委员会的成员，以及提案中具体规定的内部咨询委员会成员。这个多功能MS核心设施的建立和仪器的购买对于继续扩大和加强FUHS/CMS校园的基础研究项目至关重要。这项提议的拨款将允许这些研究人员和其他研究人员通过内部核心分析设施加强他们在结构生物学、神经退行性疾病、药物成瘾、心血管疾病和传染病方面的研究。结果在诊断、预防、基因产物的发现和验证方面具有实际应用价值。

DESCRIPTION (provided by applicant): Mass spectrometry (MS) is the choice method for complex protein sample analysis. This proposal requests the ABI QSTAR XL hybrid mass spectrometer. This vastly improved instrument contains the capabilities for both Matrix- Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) as well as an Electrospray Ionization Tandem Mass Spectrometer [ESI-MSn] to assist researchers at the Finch University of Health Sciences/Chicago Medical School (FUHS/CMS) in the areas of protein identification/characterization and proteomics/functional genomics. Currently, there is no MS capability at FUHS/CMS to satisfy the growing and urgent need for analysis of complex protein samples. The purchase of the hybrid mass spectrometer is being requested in conjunction with the establishment of the "Mass Spectrometry Core Facility", part of the Midwest Proteome Center, a unique infrastructure in the Chicago/Milwaukee region. Dr. Marc J. Glucksman, a recent recruit to CMS to establish a proteomics center, will head the MS facility. As described in the application, 14 investigators from 6 different departments on campus, funded by 7 different institutes at the NIH have a specific need for this equipment. Indeed, due to the lack of an instrument on campus, several investigators are currently forced to collaborate or contract with other institutions/companies to pursue their research goals, a situation often resulting in long delays, lower resolution data and hindered productivity. Thus, the purchase of the QSTAR XL mass spectrometer, in conjunction with the recruitment of additional faculty to establish the core facility, will have a major impact on biomedical research at CMS. A detailed organizational plan is described for efficient management and use of the instrument. The University has committed cost-sharing support for five years including the costs of setup, installation, equipment maintenance, support personnel, and a facility manager. The Principal Investigator has garnered world experts as members of the External Advisory Board of the facility, and an Internal Advisory Committee, as specified in the proposal. The establishment of this versatile MS core facility and the purchase of the instrument are essential to the continuing expansion and enhancement of the basic research programs on the FUHS/CMS campus. This proposed grant will allow these investigators and others to enhance their research in structural biology, neuro degenerative diseases, drug addiction, cardiovascular disorders, and infectious diseases with an in-house core analytical facility. The resulting work has practical application towards diagnosis, prevention, gene product discovery and validation.