LTQ Orbitrap Velos Mass Spectrometer with ETD

描述（由申请人提供）：中西部蛋白质组中心于2003年在罗莎琳德富兰克林医学与科学大学（RFUMS）/芝加哥医学院创建，以支持以质谱法为中心的蛋白质组学计划。作为一项有针对性的技术，以更新我们在结构生物学和蛋白质组学方面的研究能力，并确保最先进的仪器用于已经进行的重大扩展，RFUMS的目标是收购ThermoFisher LTQ Orbitrap Velos电子转移解离（ETD）质谱仪。除FT/ICR仪器外，Velos具有市场上最高的质量准确度、分辨率和动态范围，以及多种破碎方法。质谱法现在是中西部蛋白质组中心的常规结构分析和鉴定工具，该中心是该大学确定的许多生物医学研究计划的联系点。 该提案要求LTQ Orbitrap Velos ETD质谱仪协助15个主要用户，其中7个最近获得了NIH的新/早期资助，所有5个基础科学研究部门由NIH的10个研究所资助，需要蛋白质表征，鉴定和蛋白质组学。目前，我们NIH支持的用户超过了我们旧设备的容量和规格，其中一些将失去制造商的支持;这是提出这一要求的根本原因。而在芝加哥以南1.5小时车程的几所大学（芝加哥生物医学联盟包括美国大学。来自西北大学芝加哥的和联合（仅限于芝加哥的伊利诺斯州）在核心设施或单个实验室中拥有配备类似于此要求的仪器的旧机器，RFUMS的研究人员无法获得此关键技术。正如NIH项目描述中所详细说明的那样，我们NIH支持的研究人员和新聘教师的需求迅速扩大，需要Orbitrap Velos ETD提供更高的质量精度，分辨率，吞吐量和能力，以协助传染病，可卡因成瘾，肌肉发育，肺功能不全，神经细胞信号传导，核细胞生物学和结构生物学的研究，在这些领域中的许多领域都有辅助的蛋白质组学方法。 中西部蛋白质组中心的核心设施在2003年获得了一个QSTAR XL（QqTOF）的赞助下的S10计划，并提供了大量的“砰的一声”与补充资金的机构。8年前NCRR播下的种子在仪器曲目的扩展、用户基础的增加、获得新的/早期NIH资金的教职员工以及我们的既定研究人员利用“尖端”技术方面取得了邦蒂富尔的收获。本次提交支持的广泛学科处于NIH路线图的十字路口。拟议中的LTQ Orbitrap Velos ETD旨在满足RFUMS几乎每个基础科学部门和研究中心的用户的多样化和实现的研究需求。 公共卫生相关性：本次提交将通过在NIH十个研究所资助的不同领域提供质谱支持来增强赞助研究。15个主要用户已经注册，其中一半是新的/早期阶段的研究人员与他们的第一笔赠款。有待探讨的关键保健研究领域是：传染病、可卡因成瘾、肌肉发育、肺功能不全、脑细胞信号、核细胞生物学和膜结构生物学。

DESCRIPTION (provided by applicant): The Midwest Proteome Center was created at the Rosalind Franklin University of Medicine and Science (RFUMS)/Chicago Medical School in 2003 to bolster the proteomics initiatives centered upon mass spectrometry. As a targeted technology to update our research capabilities in structural biology and proteomics, and to insure state-of-the-art instrumentation for a major expansion already underway, RFUMS has targeted the acquisition of a ThermoFisher LTQ Orbitrap Velos with Electron Transfer Dissociation (ETD) mass spectrometer. With the exception of FT/ICR instruments, the Velos has the highest mass accuracy, resolution and dynamic range on the market, coupled with multiple fragmentation methods. Mass spectrometry is now a routine structure analysis and identification tool with the Midwest Proteome Center the nexus of many biomedical research initiatives identified by the University. This proposal requests the LTQ Orbitrap Velos ETD mass spectrometer to assist fifteen major users, seven of which have recently received New/Early stage NIH funding, in all five of the basic science research departments, funded by ten Institutes of the NIH requiring protein characterization, identification and proteomics. Currently, our NIH supported users exceed the capacity and specifications of our much older equipment, some about which are to lose manufacturer support; the fundamental reason for this request. While several universities 1.5 hours south in Chicago (the Chicago Biomedical Consortium comprising U. of Chicago, Northwestern U., and U. of Illinois in Chicago only) have older machines with an instrument similar to this request, in Core Facilities or within individual labs, researchers at RFUMS have no access to this crucial technology. As detailed in the NIH project descriptions, the rapidly expanding demands of our NIH supported researchers, and newly hired faculty, require the increased mass accuracy, resolution, throughput and capabilities afforded by the Orbitrap Velos ETD to assist studies of infectious diseases, cocaine addiction, muscle development, pulmonary insufficiency, neural cell signaling, nuclear cell biology and structural biology, with adjunct proteomic approaches to many of these areas. The Midwest Proteome Center core facility obtained a QSTAR XL (QqTOF) in 2003 under the auspices of the S10 program, and provided substantial "bang for the buck" with supplemented funds from the institution. The seed planted by the NCRR 8 years ago yielded a bountiful harvest with respect to expansion of the instrument repertoire, increased user base, faculty hires obtaining New/Early Stage NIH funding and our established investigators availing themselves of "cutting edge" technology. The wide breadth of disciplines supported by this submission is at the crossroads of the NIH Roadmap. The proposed LTQ Orbitrap Velos ETD is designed to meet the diverse and realized research needs of users in virtually every basic science department and research center at RFUMS. PUBLIC HEALTH RELEVANCE: This submission will augment sponsored research by providing mass spectrometry support in diverse areas funded by ten Institutes of the NIH. Fifteen major users have been enrolled, half of them New/Early Stage Investigators with their first grants. Crucial areas of health research to be explored are: infectious diseases, cocaine addiction, muscle development, pulmonary insufficiency, cell signaling in the brain, nuclear cell biology and membrane structural biology.