LTQ Orbitrap Velos Mass Spectrometer with ETD

描述(由申请人提供)：中西部蛋白质组中心于2003年在罗莎琳德·富兰克林医学与科学大学(RFUMS)/芝加哥医学院创建，以支持以质谱学为中心的蛋白质组学倡议。作为一项有针对性的技术，以更新我们在结构生物学和蛋白质组学方面的研究能力，并确保为正在进行的重大扩建提供最先进的仪器，RFUMS的目标是收购具有电子转移解离(ETD)功能的ThermoFisher LTQ Orbitrap Velos质谱仪。除了FT/ICR仪器外，VELOS拥有市场上最高的质量精度、分辨率和动态范围，再加上多种碎裂方法。质谱学现在是中西部蛋白质组中心的常规结构分析和鉴定工具，中西部蛋白质组中心是该大学确定的许多生物医学研究倡议的纽带。这项提案要求LTQ Orbitrap Velos ETD质谱仪在所有五个基础科学研究部门中帮助15个主要用户，其中7个用户最近获得了新的/早期阶段的NIH资助，这些资助由NIH的10个研究所资助，要求进行蛋白质表征、鉴定和蛋白质组学。目前，我们的NIH支持的用户超过了我们的旧设备的容量和规格，其中一些将失去制造商的支持；这是提出这一要求的根本原因。虽然在芝加哥以南1.5小时的几所大学(仅由芝加哥大学、西北大学和芝加哥伊利诺伊大学组成的芝加哥生物医学联盟)在核心设施或个别实验室内都有配备类似于此要求的仪器的较旧的机器，但RFUMS的研究人员无法获得这项关键技术。正如NIH项目描述中详细描述的那样，我们NIH支持的研究人员和新聘用的教师的需求迅速增长，需要Orbitrap Velos ETD提供更高的质量精度、分辨率、吞吐量和能力，以帮助研究传染病、可卡因成瘾、肌肉发育、肺功能不全、神经细胞信号、核细胞生物学和结构生物学，并辅之以蛋白质组学方法研究其中许多领域。中西部蛋白质组中心的核心设施于2003年在S10计划的支持下获得了QSTAR XL(QqTOF)，并利用该机构的补充资金提供了大量的“性价比”。NCRR在8年前播下的种子在乐器曲目的扩展、用户基础的增加、教员招聘获得新的/早期NIH资金以及我们成熟的研究人员利用“尖端”技术方面取得了丰硕成果。这份报告所支持的广泛学科处于美国国立卫生研究院路线图的十字路口。拟议的LTQ Orbitrap Velos ETD旨在满足RFUMS几乎每个基础科学系和研究中心用户的多样化和已实现的研究需求。 与公共健康相关：这份报告将通过在由美国国立卫生研究院十个研究所资助的不同领域提供质谱学支持来加强受赞助的研究。已经登记了15个主要用户，其中一半是新/早期调查人员，他们获得了第一笔赠款。有待探索的健康研究的关键领域包括：传染病、可卡因成瘾、肌肉发育、肺功能不全、脑内细胞信号、核细胞生物学和膜结构生物学。

DESCRIPTION (provided by applicant): The Midwest Proteome Center was created at the Rosalind Franklin University of Medicine and Science (RFUMS)/Chicago Medical School in 2003 to bolster the proteomics initiatives centered upon mass spectrometry. As a targeted technology to update our research capabilities in structural biology and proteomics, and to insure state-of-the-art instrumentation for a major expansion already underway, RFUMS has targeted the acquisition of a ThermoFisher LTQ Orbitrap Velos with Electron Transfer Dissociation (ETD) mass spectrometer. With the exception of FT/ICR instruments, the Velos has the highest mass accuracy, resolution and dynamic range on the market, coupled with multiple fragmentation methods. Mass spectrometry is now a routine structure analysis and identification tool with the Midwest Proteome Center the nexus of many biomedical research initiatives identified by the University. This proposal requests the LTQ Orbitrap Velos ETD mass spectrometer to assist fifteen major users, seven of which have recently received New/Early stage NIH funding, in all five of the basic science research departments, funded by ten Institutes of the NIH requiring protein characterization, identification and proteomics. Currently, our NIH supported users exceed the capacity and specifications of our much older equipment, some about which are to lose manufacturer support; the fundamental reason for this request. While several universities 1.5 hours south in Chicago (the Chicago Biomedical Consortium comprising U. of Chicago, Northwestern U., and U. of Illinois in Chicago only) have older machines with an instrument similar to this request, in Core Facilities or within individual labs, researchers at RFUMS have no access to this crucial technology. As detailed in the NIH project descriptions, the rapidly expanding demands of our NIH supported researchers, and newly hired faculty, require the increased mass accuracy, resolution, throughput and capabilities afforded by the Orbitrap Velos ETD to assist studies of infectious diseases, cocaine addiction, muscle development, pulmonary insufficiency, neural cell signaling, nuclear cell biology and structural biology, with adjunct proteomic approaches to many of these areas. The Midwest Proteome Center core facility obtained a QSTAR XL (QqTOF) in 2003 under the auspices of the S10 program, and provided substantial "bang for the buck" with supplemented funds from the institution. The seed planted by the NCRR 8 years ago yielded a bountiful harvest with respect to expansion of the instrument repertoire, increased user base, faculty hires obtaining New/Early Stage NIH funding and our established investigators availing themselves of "cutting edge" technology. The wide breadth of disciplines supported by this submission is at the crossroads of the NIH Roadmap. The proposed LTQ Orbitrap Velos ETD is designed to meet the diverse and realized research needs of users in virtually every basic science department and research center at RFUMS. PUBLIC HEALTH RELEVANCE: This submission will augment sponsored research by providing mass spectrometry support in diverse areas funded by ten Institutes of the NIH. Fifteen major users have been enrolled, half of them New/Early Stage Investigators with their first grants. Crucial areas of health research to be explored are: infectious diseases, cocaine addiction, muscle development, pulmonary insufficiency, cell signaling in the brain, nuclear cell biology and membrane structural biology.