LC-Tandem Mass Spectrometer

LC-串联质谱仪

• 批准号: 6733482
• 负责人: ROBERT MARTIN BLUMENTHAL
• 金额: $ 28.13万
• 依托单位: UNIVERSITY OF TOLEDO HEALTH SCI CAMPUS
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6733482
• 关键词: biomedical equipment purchase; liquid chromatography mass spectrometry; protein quantitation /detection; proteomics

DESCRIPTION (provided by applicant): Understanding how genomes control the physiology of biological systems requires detailed analysis of the proteins produced. In the past several years, technical refinements to mass spectroscopy and bioinformatics methods have increased the power of analyzing protein populations (proteomics) by another order of magnitude, making it vital to research projects at the Medical College of Ohio and neighboring institutions. The purpose of this proposal is to obtain a liquid chromatography - tandem mass spectroscopy (LC/MS/MS) instrument, along with the core accessories required to allow its most efficient use by NIH-funded major users, and a variety of minor users from the Medical College of Ohio, University of Toledo, and Bowling Green State University. Unlike DNA sequencing, efficient proteomic analysis by LC/MS/MS requires close interactions between the research team and core laboratory personnel. This is particularly true of LC/MS/MS, in which a population of tryptic peptides are resolved, released from a gated ion trap, fragmented by collision with atoms of an inert gas, and sequenced by analyzing of the mass fingerprint of the resulting ions. Laboratory space to house the proposed equipment, and expertise required to ensure its optimal use, are already in place at the Medical College of Ohio. Major and minor users of the proposed LC/MS/MS equipment have been identified. The NIH-funded projects of the eight major users include characterization of the fungal pathogen and potential bioterrorism agent Coccidioides immitis, the link between reactive oxygen species-dependent inactivation of Na + ATPase and pathological changes in cardiac myocytes, characterization of proteins interacting with Na + ATPase, the role of the CEACAM1 cell adhesion molecule in insulin metabolism, regulation of replication of the potential gene therapy vector adeno-associated virus (AAV), characterization of the MAP kinase inhibitory protein RKIP, investigating the role of Rab GTPases in controlling cell growth, and characterizing the endocytosis and recycling of the GPI-anchored folate receptor. All of these projects, and others as well, will be substantially strengthened by the requested equipment.

描述（由申请人提供）：了解基因组如何控制生物系统的生理学需要对产生的蛋白质进行详细分析。在过去的几年里，质谱和生物信息学方法的技术改进使分析蛋白质群体（蛋白质组学）的能力又提高了一个数量级，这对俄亥俄州医学院和邻近机构的研究项目至关重要。本提案的目的是获得液相色谱-串联质谱（LC/MS/MS）仪器，沿着允许NIH资助的主要用户最有效使用所需的核心配件，以及来自俄亥俄州医学院、托莱多大学和鲍林绿色州立大学的各种次要用户。与DNA测序不同，LC/MS/MS的高效蛋白质组学分析需要研究团队和核心实验室人员之间的密切互动。LC/MS/MS尤其如此，其中胰蛋白酶肽的群体被解析，从门控离子阱释放，通过与惰性气体的原子碰撞而碎片化，并通过分析所得离子的质量指纹来测序。俄亥俄州医学院已经有了容纳拟议设备的实验室空间和确保其最佳使用所需的专业知识。 已确定拟议LC/MS/MS设备的主要和次要用户。八个主要用户的NIH资助项目包括真菌病原体和潜在生物恐怖剂粗球孢子菌的表征，Na + ATP酶活性氧依赖性失活与心肌细胞病理变化之间的联系，与Na + ATP酶相互作用的蛋白质的表征，CEACAM 1细胞粘附分子在胰岛素代谢中的作用，潜在基因治疗载体腺相关病毒（AAV）复制的调节，MAP激酶抑制蛋白RKIP的表征，Rab GTP酶在控制细胞生长中的作用的研究，以及GPI锚定的叶酸受体的内吞作用和再循环的表征。所有这些项目以及其他项目都将因所要求的设备而得到大大加强。