Inverted Laser-Scanning Confocal Microscope

倒置激光扫描共焦显微镜

• 批准号: 6730774
• 负责人: Douglas J. Taatjes
• 金额: $ 47.34万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6730774
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; confocal scanning microscopy

DESCRIPTION (provided by applicant): The goal of this proposal is to obtain a new state-of-the-art inverted confocal microscope to augment the heavily used upright confocal microscope in our Microscopy Imaging Center (MIC). This instrument would meet the increasing demand for advanced features not supported by the existing nine year-old microscope and would sustain the need for efficient confocal imaging for years to come with a reliable, high performance system. Importantly, this microscope will permit extended live cell and tissue imaging for short- and longterm physiological experiments. The requested instrument will be essential for future cutting edge biomedical research for the UVM College of Medicine community, and a vital adjunct to the MIC. The rationale for a new system is to enhance and sustain our current confocal imaging capabilities with a broader range of sophisticated imaging applications. A modern scanning confocal system will also meet the progressively increasing demand for specialized confocal imaging, especially for investigations using live cells and thick tissues, FRET/FRAP analyses, and simultaneous multi-channel imaging. Five laboratories have been identified as major users for the purpose of this application because they represent most of the projected demand for the requested instrument given their existing and proposed applications requirements. We have also identified a strong minor user group that will occupy the remaining time on the proposed new system. All are well-funded investigators with PHS grants. The addition of a new inverted confocal microscope to the MIC is expected to significantly absorb the progressively expanding numbers of users of the facility and meet their specific technical requirements. It will also afford the capacity for advanced high-quality imaging capabilities and ensure sustained and efficient use for the UVM biomedical research community for the future.

描述（由申请人提供）：该提案的目的是获得新的最先进的倒置共聚焦显微镜，以增强我们的显微镜成像中心（MIC）中大量使用的直立共聚焦显微镜。该工具将满足现有九年历史的显微镜不支持的对高级功能的不断增长的需求，并将维持多年来有效共聚焦成像的需求，并具有可靠的高性能系统。重要的是，该显微镜将允许长期生理实验的扩展活细胞和组织成像。该要求的仪器对于UVM医学学院的将来的尖端生物医学研究至关重要，并且是麦克风的重要辅助手段。新系统的理由是通过更广泛的复杂成像应用来增强和维持我们当前的共聚焦成像功能。现代的扫描共聚焦系统还将满足对专业共聚焦成像的逐渐增加的需求，尤其是用于使用活细胞和较厚组织，FRET/FRAP分析以及同时进行多通道成像的研究。出于本应用程序的目的，五个实验室被确定为主要用户 鉴于其现有和拟议 应用要求。我们还确定了一个强大的次要用户组，该用户组将占据拟议的新系统上的剩余时间。所有人都是资金丰富的调查员，并获得了PHS补助金。预计将新的倒置共聚焦显微镜添加到麦克风中会显着吸收该设施的用户数量逐渐扩大并满足其特定的技术要求。它还将负担高级高质量成像能力的能力，并确保未来UVM生物医学研究界的持续有效使用。

项目成果

Multifluorescence confocal microscopy: application for a quantitative analysis of hemostatic proteins in human venous valves.

• DOI: 10.1007/978-1-62703-056-4_4
• 发表时间: 2013-01-01
• 期刊: Methods in molecular biology (Clifton, N.J.)
• 作者: Trotman, Winifred E;Taatjes, Douglas J;Bovill, Edwin G
• 通讯作者: Bovill, Edwin G

Actin polymerization plays a significant role in asbestos-induced inflammasome activation in mesothelial cells in vitro.

• DOI: 10.1007/s00418-016-1530-8
• 发表时间: 2017-05
• 期刊: Histochemistry and cell biology
• 影响因子: 2.3
• 作者: MacPherson M;Westbom C;Kogan H;Shukla A
• 通讯作者: Shukla A

A novel combined imaging/morphometrical method for the analysis of human sural nerve biopsies for clinical diagnosis.

一种新颖的组合成像/形态测量方法，用于分析人类腓肠神经活检以进行临床诊断。

• DOI: 10.1007/978-1-62703-056-4_19
• 发表时间: 2013
• 期刊: Methods in molecular biology (Clifton, N.J.)
• 作者: Vonturkovich,MicheleA;Wadsworth,MarilynP;Pendlebury,WilliamW;Taatjes,DouglasJ
• 通讯作者: Taatjes,DouglasJ