Capillary DNA Sequencers for the OUHSC Core Facility

描述(由申请人提供)：俄克拉荷马大学健康科学中心(OUHSC)微生物基因组学实验室拥有一个核心DNA测序设施，这是一个大学建立的服务单位，负责为OUHSC社区提供DNA测序、人类白细胞抗原配型、高通量寡核苷酸合成、微阵列制造、杂交和分析以及生物信息学支持。在行政上，这一核心设施设在微生物学和免疫学系内，由John Iandolo博士担任主席；该部负责对核心设施进行预算和受托监督。机构对这一核心设施的承诺始于1995年，当时OUHSC承诺与最初建立核心设施的NSF设备赠款50%匹配，购买了三台Pharmacia ALF Express平板凝胶DNA测序仪，这三台测序仪目前用于人类白细胞抗原分型。在其间的几年中，该机构为购买两台ABI 377平板凝胶测序仪、一台ABI 3700毛细DNA测序仪提供了支持，为第二台ABI 3700提供了部分支持，并为购买其他设备提供了额外资金，包括一台带有RAID阵列和外部磁带备份的Sun Enterprise 450服务器、一台基因组解决方案Flexsys阵列打印机/菌落拾取机器人，以及一台BioAutomation MerMade多重寡核苷酸合成仪的配套资金。加上NIH和NSF的其他拨款以及OUHSC多年来提供的额外资金，该大学已为这一核心设施投资了远远超过100万美元的资本设备。由于这一设施的成功，我们园区对DNA测序服务的需求已经增长到现有设施在没有额外设备的情况下无法继续支持这一需求的地步。具体地说，我们建议购买：1)ABI3100 16毛细管DNA测序仪，以取代ABI377平板凝胶测序仪，增加产量，削减成本，增强数据质量，并缓解现有测序服务的瓶颈，这会减缓小项目DNA测序的速度；2)ABI3730 48毛细管DNA测序仪，取代原来的Pharmacia ALF快速测序仪，将我们的HLA分型能力提升到毛细管方法，从而增加产量和削减成本。

DESCRIPTION (provided by applicant): The Oklahoma University Health Sciences Center (OUHSC) Laboratory for Microbial Genomics houses a core DNA sequencing facility that is a university-established service unit, responsible for providing DNA sequencing, HLA typing, high-throughput oligonucleotide synthesis, microarray fabrication, hybridization and analysis, and bioinformatics support to the OUHSC community. Administratively, this core facility is housed within the Department of Microbiology and Immunology, chaired by Dr. John Iandolo; the department has the responsibility for budgetary and fiduciary oversight of the core facility. The institutional commitment to this core facility began in 1995, when OUHSC committed a 50% match to an NSF equipment grant that originally established the core facility, by the purchase of three Pharmacia ALF Express slab gel DNA sequencers, which are currently used for HLA typing. In the intervening years, the institution has provided support for the purchase of two ABI 377 slab gel sequencers, one ABI 3700 capillary DNA sequencer, partial support for a second ABI 3700, and additional funds for the purchase of other equipment including a Sun Enterprise 450 server with RAID array and external tape backup, a Genomic Solutions Flexsys array printer/colony picker robot, and matching funds for a Bioautomation MerMade multiplex oligonucleotide synthesizer. With other NIH and NSF grants and additional funds provided by OUHSC over the years, the university has invested well over $1 million in capital equipment for this core facility. Because of the success of this facility, the demand for DNA sequencing services on our campus has grown to the point where the existing facility cannot continue to support this demand without additional equipment. Specifically, we propose to purchase: 1) an ABI3100 16-capillary DNA sequencer to replace the ABI377 slab gel sequencers, increase throughput, cut costs, enhance data quality, and relieve the existing bottleneck on sequencing service that slows DNA sequencing for small projects, and 2) an ABI3730 48-capillary DNA sequencer, replacing the original Pharmacia ALF express sequencers to upgrade our HLA typing capacity to capillary methodology, increasing throughput and cutting costs.