Core--Specimen

描述(由申请人提供)：获取具有良好特征的标本和实验室 专业知识对于该孢子计划中提出的每一项研究的成功，以及卵巢癌转化性研究的长期成功至关重要。这个核心将与所有孢子核心、项目和发展研究协调工作，以实现计划目标。标本核心将继续收集、处理、鉴定、存储和跟踪组织、血液样本和其他独特的样本，扩大目前的储存库内容，用于孢子项目和发展研究研究。我们有组织和全面的标本收集和鉴定方法包括：(A)一个系统的标本收集过程；(B)审查，以确保病理分析的质量和一致性；(C)根据熟悉POCRC所有研究工作的标本审查委员会确定的优先顺序，对标本进行集中管理，以促进分发。与该核心相关的研究实验室为孢子研究项目提供关键服务，包括病理学审查、抗体开发、原代卵巢癌细胞培养和细胞系的建立和鉴定，以及及时进行 生物标记物小组验证。至关重要的是，样品芯继续进化，以满足 我们孢子项目不断变化的需求。为此，该核心将强调最大限度地提高效率和满足项目需求的协议。例如，将血液产品加工成0.3毫升的血清和血浆等份可消除有害的冻融循环，并确保早期检测项目收到用于生物标记物评估的高质量样本。此外，将原代细胞培养添加到标本处理方案中，有助于免疫治疗和分子靶标项目的工作。标本核心与其他三个核心一起工作，将确保所有项目，包括发展研究，都能获得广泛的标本和数据。最后，虽然建立标本核心主要是为了满足孢子项目的需要，但标本核心资源的创建具有足够的灵活性，因此孢子合作者也可以利用这一非常重要的科学核心共享资源。因此，外部研究人员和其他孢子也可以通过新的虚拟共享标本获得标本清单 存储库(VSSR)。

DESCRIPTION (provided by applicant): Access to well-characterized specimens and laboratory expertise is crucial to the success of each research study proposed within this SPORE program, and to the long-term success of translational research in ovarian cancer. This core will work in concert with all SPORE Cores, Projects and Developmental Research Studies to achieve program goals. The Specimen Core will continue to collect, process, characterize, store, and track tissue, blood samples and other unique specimens, expanding current repository contents for use in SPORE projects and developmental research studies. Our organized and comprehensive approach to specimen accrual and characterization includes (a) a systematic process for specimen collection (b) review to assure quality and consistency in pathologic analyses and (c) centralized management of specimens to facilitate distribution based on priorities defined by a Specimen Review Committee familiar with all POCRC research endeavors. Research laboratories associated with this Core provide critical services to SPORE research projects, including pathology review, antibody development, establishment and characterization of primary ovarian cancer cell cultures and cell lines, and conduct of timely assays for biomarker panel validation. It is essential that the Specimen Core continue to evolve to meet the changing needs of our SPORE program. Towards that end, this Core will emphasize protocols that maximize efficiency and fulfill project needs. For example, processing blood products into 0.3 ml serum and plasma aliquots eliminates harmful freeze-thaw cycles and ensures that the Early Detection Project receives high quality specimens for biomarker evaluation. Furthermore, addition of primary cell cultures to specimen processing protocols facilitates the work of the Immunotherapy and Molecular Targets Projects. Working in conjunction with the other three Cores, the Specimen Core will ensure that all projects, including developmental research studies, have access to a broad spectrum of specimens and data. Lastly, while established to primarily serve the needs of the SPORE projects, the resources of the Specimen Core have been created with sufficient flexibility such that SPORE collaborators may also utilize this very important scientific Core shared resource. Accordingly, specimen inventories will also be available to outside researchers and to other SPOREs through the new Virtual Shared Specimen Repository (VSSR).