Core-Electron Spin Resonance

核心A的目的是提供最先进的活性氧（ROS）和一氧化氮（NO）的测量帮助。在血管细胞和组织中的作用。CORE A将协助制备和处理各种生物样品，以便使用电子自旋共振（ESR）光谱进行分析。具有自旋捕获技术的ESR光谱是即使在使用最少量生物材料的强散射介质中也能高灵敏度定量检测活性氧的最直接和明确的方法之一。该核心设施自1999年以来一直存在，并支持超氧自由基的研究（02.-）和NO。 Griendling and Harrison（1- 10）.该设施将显著增强各个实验室的工作，并将明显改善PPG参与者之间的合作。 具体而言，电子自旋共振核心将： 1.提供专业知识，设施，并为个人进行测量ROS和NO的培训。使用ESR光谱。 2.为PPG参与者提供最先进的血管细胞和组织中ROS和NO的体外和离体测量帮助。 3.开发技术和完善现有的方法来测量血管组织中的ROS和NO。

The purpose of Core A is to provide assistance with state-of-the-art measurements of reactive oxygen species (ROS) and nitric oxide (NO.) in vascular cells and tissues for the participants of the PPG. CORE A will assist with preparation and processing of various biological samples for analysis using Electron Spin Resonance (ESR) spectroscopy. ESR spectroscopy with a spin trapping technique is one of the most direct and unequivocal methods for high sensitivity quantitative detection of reactive oxygen species even in strongly scattering media using minimal amounts of biological material. This Core facility has existed since 1999 and has supported studies of superoxide radical (02.-) and NO. in vascular cells in several publications from Drs. Griendling and Harrison (1- 10). This facility will significantly enhance the work of the individual labs and will clearly improve collaborations between participants of the PPG. Specifically, the Electron Spin Resonance Core will: 1. Offer expertise, facilities, and training for individuals in performing measurements of ROS and NO. using ESR spectroscopy. 2. Provide assistance with state-of-the-art in vitro and ex vivo measurements of ROS and NO. in vascular cells and tissues for participants of the PPG. 3. Develop techniques and refine existing methods for measurement of ROS and NO. in vascular tissues.