REACTIVE OXYGEN SPECIES CORE

项目总结 活性氧物种核心A的目的是提供最先进的测量 活性氧种(ROS)，识别ROS的来源，并协助在完整细胞和 PPG参与者的体外和体内细胞组分。核心A将协助准备和 使用电子自旋共振(ESR)光谱处理用于分析的各种生物样品， 高效液相色谱和荧光光谱分析。该核心设施拥有  支持了大量关于血管细胞中超氧化物歧化酶(O2)、过氧化氢(H2O2)和一氧化氮的研究 组织。这一设施将大大加强各个实验室的工作，并将增强 PPG参与者之间的协作。具体地说，核心A将： 1.为个人提供专业知识、设施和培训，以进行ROS、NO和 过氧亚硝酸根的ESR波谱、高效液相色谱和荧光光谱。 2.提供最先进的体外、体外和体内ROS、NO和 PPG参与者细胞和组织中的过氧亚硝酸盐。 3.开发技术和改进现有的细胞内ROS、NO和过氧亚硝酸根的测量方法 还有纸巾。 核心领导者迪卡洛夫博士是体外和体外测量血管中ROS和NO的专家 在开发新的ROS检测方法方面一直走在前列。大部分 他的工作一直集中在开发基于ESR和高效液相的体外和体内新技术 ROS的测量。活性氧物种核心A将开发新方法并精炼现有 使用基于硼酸盐的ESR探针测量细胞和相关组织中的ROS的技术 针对特定细胞内的过氧亚硝酸盐荧光探针和氧化还原敏感的GPF荧光蛋白 细胞器。这些方法的应用将提高个别项目负责人的测试能力 他们的假设，并根据需要设计新的研究来推进他们的工作。

PROJECT SUMMARY The purpose of REACTIVE OXYGEN SPECIES CORE A is to provide state-of-the-art measurements of reactive oxygen species (ROS), identify the sources of ROS and assist with ROS studies in intact cells and cellular fractions, ex vivo and in vivo for participants of the PPG. Core A will assist with preparation and processing of various biological samples for analysis using Electron Spin Resonance (ESR) spectroscopy, High Performance Liquid Chromatography (HPLC) and fluorescent spectroscopy. This Core facility has  supported numerous studies of superoxide (O2 ), hydrogen peroxide (H2O2) and NO in vascular cells and tissue. This facility will significantly enhance the work of the individual laboratories and will enhance collaborations between participants of the PPG. Specifically, the Core A will: 1. Offer expertise, facilities, and training for individuals in performing measurements of ROS, NO and peroxynitrite using ESR spectroscopy, HPLC and fluorescent spectroscopy. 2. Provide assistance with state-of-the-art in vitro, ex vivo and in vivo measurements of ROS, NO and peroxynitrite in cells and tissues for participants of the PPG. 3. Develop techniques and refine existing methods for measurement of ROS, NO and peroxynitrite in cells and tissues. The core leader, Dr. Dikalov, is an expert in in vitro and ex vivo measurements of ROS and NO in vascular cells and tissues and has been at the forefront in developing new methodologies for ROS detection. Much of his work has been focused on development of new ESR and HPLC based techniques for in vitro and in vivo measurements of ROS. REACTIVE OXYGEN SPECIES CORE A will develop new methods and refine existing techniques for measurements of ROS in cells and relevant tissues using ESR probes, boronate-based peroxynitrite fluorescent probes and redox sensitive GPF fluorescent protein targeted to specific intracellular organelles. The application of these methods will enhance the ability of the individual project leaders to test their hypotheses and design new studies as necessary to advance their work.