Core--Neuropathology and Cell Culture

神经病理学/细胞培养核心(核心B)在我们的计划项目中起着核心作用。Core B的方法将是多学科的，使用神经病理学和细胞培养方法来评估缺血性脑损伤的机制。有两个主要目标。在第一个目标中，核心B的工作人员将负责准备局灶性和全局性缺血研究的所有组织切片，包括梗塞体积测定、免疫组织化学、DNA断裂染色(TUNEL)和原位杂交处理的切片。该工作人员将协助培训相关数据分析项目1-5的人员。在第二个目标中，核心B的员工 将为项目1、4和5准备和维护所有原代细胞培养。CORE B工作人员还将执行不同的诱导毒性模型，并对培养细胞进行免疫细胞化学、体外受体放射自显影、细胞结合分析和药物治疗。工作人员将协助培训各个项目中与细胞培养相关的数据分析方面的人员。

The Neuropathology/Cell Culture Core (Core B) has a central role in our Program Project. The approach of Core B will be multidisciplinary, using neuropathological and cell culture methods to evaluate mechanisms of ischemic brain injury. There are two main aims. In the first aim, the staff of Core B will be responsible for preparing all histologic sections for studies of focal and global ischemia, including sections processed for infarct volume determination, immunohistochemistry, DNA break staining (TUNEL), and in situ hybridization. The staff will assist in training personnel from Projects 1-5 in the associated data analysis. In the second aim, the staffof Core B will prepare and maintain all primary cell cultures for projects 1, 4, and 5.). Core B staff will also perform different models of induced-toxicity and perform immunocytochemistry, in vitro receptor autoradiography, cell binding assays, and pharmacological treatments on cultured cells. The staff will assist in training personnel from the various Projects in the data analysis associated with the cell cultures.