Core--Neuropathology and Cell Culture

神经病理学/细胞培养核心（核心B）在我们的项目中起着核心作用。核心B的方法将是多学科的，使用神经病理学和细胞培养方法来评估缺血性脑损伤的机制。有两个主要目的。在第一个目标中，Core B的工作人员将负责准备局灶性和全局性缺血研究的所有组织学切片，包括用于梗死体积测定、免疫组织化学、DNA断裂染色（TUNEL）和原位杂交的切片。工作人员将协助培训项目1-5人员进行相关数据分析。在第二个目标中，B核心的员工

The Neuropathology/Cell Culture Core (Core B) has a central role in our Program Project. The approach of Core B will be multidisciplinary, using neuropathological and cell culture methods to evaluate mechanisms of ischemic brain injury. There are two main aims. In the first aim, the staff of Core B will be responsible for preparing all histologic sections for studies of focal and global ischemia, including sections processed for infarct volume determination, immunohistochemistry, DNA break staining (TUNEL), and in situ hybridization. The staff will assist in training personnel from Projects 1-5 in the associated data analysis. In the second aim, the staffof Core B will prepare and maintain all primary cell cultures for projects 1, 4, and 5.). Core B staff will also perform different models of induced-toxicity and perform immunocytochemistry, in vitro receptor autoradiography, cell binding assays, and pharmacological treatments on cultured cells. The staff will assist in training personnel from the various Projects in the data analysis associated with the cell cultures.