Core--Neuropathology and Cell Culture

神经病理学/细胞培养核心（核心B）在我们的计划项目中发挥着核心作用。核心B的方法将是多学科的，使用神经病理学和细胞培养方法来评估缺血性脑损伤的机制。有两个主要目标。在第一个目标中，核心B的工作人员将负责制备用于局灶性和全脑缺血研究的所有组织学切片，包括用于梗死体积测定、免疫组织化学、DNA断裂染色（TUNEL）和原位杂交的处理切片。工作人员将协助培训项目1-5的人员进行相关的数据分析。在第二个目标中，核心B的员工 将为项目1、4和5制备和维护所有原代细胞培养物）。核心B工作人员还将进行不同的诱导毒性模型，并进行免疫细胞化学，体外受体放射自显影，细胞结合试验和对培养细胞的药物治疗。工作人员将协助培训来自不同项目的人员进行与细胞培养相关的数据分析。

The Neuropathology/Cell Culture Core (Core B) has a central role in our Program Project. The approach of Core B will be multidisciplinary, using neuropathological and cell culture methods to evaluate mechanisms of ischemic brain injury. There are two main aims. In the first aim, the staff of Core B will be responsible for preparing all histologic sections for studies of focal and global ischemia, including sections processed for infarct volume determination, immunohistochemistry, DNA break staining (TUNEL), and in situ hybridization. The staff will assist in training personnel from Projects 1-5 in the associated data analysis. In the second aim, the staffof Core B will prepare and maintain all primary cell cultures for projects 1, 4, and 5.). Core B staff will also perform different models of induced-toxicity and perform immunocytochemistry, in vitro receptor autoradiography, cell binding assays, and pharmacological treatments on cultured cells. The staff will assist in training personnel from the various Projects in the data analysis associated with the cell cultures.