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OPTOELECTRONIC NANOWIRE PROBES FOR INVESTIGATION OF INTRA-CELLULAR PROCESSES

用于研究细胞内过程的光电纳米线探针

基本信息

批准号：
7981652
负责人：
Ritesh Agarwal
金额：
$ 240万
依托单位：
UNIVERSITY OF PENNSYLVANIA
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-09-30 至 2015-06-30
项目状态：
已结题

项目摘要

DESCRIPTION (Provided by the applicant) Abstract: We propose to assemble nanowire devices with optoelectronic and electrical functionalities to probe intra- and inter-cellular dynamics with unprecedented spatial and temporal resolution. Arrays of electrically pumped nanowire waveguides, lasers, light emitting diodes, and photodetectors combined along with their ability to function as nanoelectrodes will be utilized to probe organelles and other subcellular targets with nanoscale resolution and measure in real-time chemical reaction kinetics, signal propagation, and reactions due to a locally delivered drug, amongst other complex phenomena occurring over any relevant length scales. The arrays of nanowire probes will be functionalized with fluorophores, quantum dots, plasmonic nanocrystals, either at their tips or on their surfaces to enable almost any type of biological imaging technique. Using a general nanowire probe platform will lead to the development of a very broad set of tools with novel functionalities, to enable imaging and electrical probing with label-free techniques with the unique capability of probing any desired spatial domain within living cells. These nanowire-probe substrates can be easily integrated with AFM cantilevers or the tips of conventional fibers, which can then be combined with standard 3-D nanopositioning systems, external electrical circuitry, and optical microscopes to probe specific domains of intracellular organelles/components. As an example, these nanowire devices will be used to create novel nanoscale interfaces with neurons and neuronal networks in the hippocampus to study neuronal signal integration and network functioning and then utilized to investigate the pathophysiology of diseases such as epilepsy. The integration of optoelectronic and electrical functionalities of nanowires on a common platform would lead to a new generation of nanosystems with unprecedented sensitivity and selectivity in probing subcompartments of living cells at the molecular level, which could revolutionize our knowledge of these biological systems and tremendously aid in future drug discoveries. Public Health Relevance: The ability to visualize in vitro intra- and inter- cellular processes in real time with multiplexed and nanoscale resolution detection with the proposed combined optoelectronic and electrical nanowire probes will elucidate new chemical and electrochemical processes and signaling pathways. Detailed knowledge of suband inter- cellular processes using nanowire probes will lead to a much better understanding of overall cellular processes and will aid the design of new drugs for a large number of diseases thereby impacting public health.

描述（由申请人提供）摘要：我们建议组装具有光电和电学功能的纳米线器件，以前所未有的空间和时间分辨率探测细胞内和细胞间的动力学。电泵浦纳米线波导、激光器、发光二极管和光电探测器的阵列及其作为纳米电极的功能将被用来以纳米级分辨率探测细胞器和其他亚细胞靶标，并测量实时化学反应动力学、信号传播和局部递送药物引起的反应，以及任何相关的发生的其他复杂现象。长度尺度。纳米线探针阵列将在其尖端或表面上用荧光团、量子点、等离子体纳米晶体进行功能化，以实现几乎任何类型的生物成像技术。使用通用纳米线探针平台将导致开发出一系列具有新颖功能的非常广泛的工具，以利用无标记技术进行成像和电探测，并具有探测活细胞内任何所需空间域的独特能力。这些纳米线探针基底可以轻松地与 AFM 悬臂或传统纤维的尖端集成，然后可以与标准 3D 纳米定位系统、外部电路和光学显微镜相结合，以探测细胞内细胞器/组件的特定区域。例如，这些纳米线设备将用于与海马体中的神经元和神经元网络创建新型纳米级接口，以研究神经元信号整合和网络功能，然后用于研究癫痫等疾病的病理生理学。将纳米线的光电和电功能集成在一个通用平台上将产生新一代纳米系统，在分子水平上探测活细胞的子区室时具有前所未有的灵敏度和选择性，这可能会彻底改变我们对这些生物系统的认识，并极大地有助于未来的药物发现。公共健康相关性：通过使用所提出的组合光电和电纳米线探针进行多重和纳米级分辨率检测，实时可视化体外细胞内和细胞间过程的能力将阐明新的化学和电化学过程和信号传导途径。使用纳米线探针对细胞亚和细胞间过程的详细了解将有助于更好地理解整个细胞过程，并将有助于设计针对大量疾病的新药，从而影响公共健康。