Holding the front line - maintaining barrier integrity in the mouth

坚守第一线——保持口腔屏障完整性

• 批准号: 2619605
• 金额: --
• 依托单位: University of Glasgow
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2619605
• 关键词: Holding; front; line; maintaining; barrier

Studentship strategic priority area: Basic Bioscience Underpinning HeathKeywords: Oral, mucosa, immunity, host-microbe The gums in the mouth (gingivae) are constantly exposed to food, bacteria, and damage from chewing. The immune system must keep the gums healthy - despite the bacteria and damage from chewing. When this protection system fails, the barrier no longer functions, and this causes poor dental and general health. Compared with other barrier sites such as the gut, the mechanisms that keep the gingivae healthy are relatively poorly understood. Understanding how the immune system maintains healthy gums will provide new understanding of how oral health is maintained, and how effective maintenance of oral health may also prevent other diseases that affect general health. The team in Glasgow have established system to grow the bacteria that live in the mouth in the laboratory - and these lab-grown bacteria mimic the communities of bacteria found in the mouth. The bacteria can be set up alongside gingival tissue to establish how different bacteria impact the gingivae. This project aims to find out how the immune system works in the gingivae, and how the bacteria work alongside this immune system to keep the mouth healthy. This work aligns with several key BBSRC priorities. These studies will provide a change in our understanding of the influence of the microbiome on the host immune system. At least 10% of all antibiotic prescriptions provided in healthcare are prescribed for dental problems. Better understanding how to keep the mouth healthy can help prevent dental diseases and therefore in the long term reduce the reliance on antibiotics. Understanding the interactions between bacteria and the immune response may highlight that there are alternatives to antibiotics that can restore health. Thus, this project can help move towards alternatives to antimicrobials for dental health. The project will develop a model system that provides insights into physiological processes that are key for maintaining health - thereby informing healthy aging across the life course. The work will involve carefully validated in vitro model systems, that will further the absolute reduction of use of animals in research. The student working on this project will receive extensive training in a range of laboratory methods, in particular working with bacteria and cells of the immune system. The student will be involved in a wide variety of cutting-edge methods of data gathering and data analysis and will be working in a team of enthusiastic and highly motivated PhD students and post-doctoral researchers. In addition to skills specific to the project, there is training in a wide variety of skills that are essential for a scientist including presentations, writing, communication, ethics, regulatory matters, and data management among others. The student will work with our industry partner to gain exceptionally valuable experience in technology transfer, product development and marketing. Previous students from the group have gone on to successful roles in academia, industry, education, and communications.

学生战略优先领域：基础生物科学支撑健康关键词：口腔，粘膜，免疫，宿主微生物口腔中的牙龈（牙龈）不断暴露于食物，细菌和咀嚼损伤。免疫系统必须保持牙龈健康-尽管有细菌和咀嚼造成的损害。当这种保护系统失效时，屏障不再起作用，这会导致牙齿和一般健康状况不佳。与肠道等其他屏障部位相比，保持牙龈健康的机制相对知之甚少。了解免疫系统如何维持健康的牙龈将提供对口腔健康如何维持的新理解，以及如何有效地维持口腔健康也可以预防影响一般健康的其他疾病。格拉斯哥的研究小组已经建立了在实验室中培养口腔细菌的系统，这些实验室培养的细菌模仿了口腔中的细菌群落。细菌可以设置在牙龈组织旁边，以确定不同的细菌如何影响牙龈。该项目旨在了解免疫系统如何在牙龈中工作，以及细菌如何与免疫系统一起工作以保持口腔健康。这项工作符合BBSRC的几个关键优先事项。这些研究将改变我们对微生物组对宿主免疫系统影响的理解。在医疗保健中提供的所有抗生素处方中，至少有10%是用于牙齿问题的。更好地了解如何保持口腔健康可以帮助预防牙科疾病，因此从长远来看可以减少对抗生素的依赖。了解细菌和免疫反应之间的相互作用可能会突出表明，有抗生素的替代品可以恢复健康。因此，该项目可以帮助转向用于牙齿健康的抗菌剂替代品。该项目将开发一个模型系统，提供对维持健康的关键生理过程的见解，从而在整个生命过程中为健康老龄化提供信息。这项工作将涉及经过仔细验证的体外模型系统，这将进一步减少研究中使用动物的绝对数量。从事该项目的学生将接受一系列实验室方法的广泛培训，特别是与细菌和免疫系统细胞一起工作。学生将参与各种各样的数据收集和数据分析的尖端方法，并将在一个充满热情和高度积极性的博士生和博士后研究人员团队中工作。除了特定于该项目的技能外，还有各种技能的培训，这些技能对科学家来说是必不可少的，包括演讲，写作，沟通，道德，监管事项和数据管理等。学生将与我们的行业合作伙伴合作，在技术转让，产品开发和营销方面获得非常宝贵的经验。以前的学生从该集团已经在学术界，工业，教育和通信成功的角色。