Ultra-sensitive magnetic assays for sepsis diagnostics

用于脓毒症诊断的超灵敏磁性测定

• 批准号: 7094247
• 负责人: Mark S. DiIorio
• 金额: $ 89.48万
• 依托单位: MAGNESENSORS, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-15 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7094247
• 关键词: Escherichia coli; Staphylococcus aureus; biomedical equipment development; clinical biomedical equipment; clinical research; diagnosis design /evaluation; early diagnosis; human tissue; magnetic field; rapid diagnosis; septicemia

DESCRIPTION (provided by applicant): The program is aimed at applying an ultra-sensitive magnetic detection platform to the rapid and early diagnosis of pathogens causing sepsis. The technology platform is based on labeling biomolecules with magnetic nanoparticles and detecting the magnetic field generated upon binding using patented ultra-sensitive magnetic sensors. While the initial focus will be on a "proof-of-principle" demonstration for the direct detection of two major pathogens, Staphylococcus aureus and Escherichia coli, responsible for sepsis, the diagnostic platform should be generally applicable to a wide range of pathogens. Direct detection of live bacteria will be demonstrated by measuring the binding of magnetic labeled antibodies to a surface antigen on the target pathogen. Purchased bacteria will first be used to demonstrate a proof-of-concept for high sensitivity in a rapid, "mix and measure" magnetic assay. A next generation prototype instrument will then be produced and placed at our collaborator, Children's Hospital Boston. An existing IRB-approved library of clinical specimens with documented infections (600 blood and urine samples, 150 trachea) aspirates) will be examined in order to demonstrate clinical utility, including sensitivity, specificity and short assay times. The ultimate goal of this program is to show a limit of detection < 100 bacteria/ml in clinical samples with a total (preparation and measurement) assay time of less than 15 minutes. Additionally, detection limits as low as 5-10 bacteria in small samples (e.g., 20 ul) may be possible by using the unique ability to externally manipulate magnetic nanoparticle labels using magnetic fields. Note that magnetic detection is not hampered by measurement in biological fluids and the mix and measure format in microplate wells requires little sample preparation, thereby minimizing errors.

描述(申请人提供)：该项目旨在将超灵敏磁性检测平台应用于导致败血症的病原体的快速和早期诊断。该技术平台的基础是用磁性纳米颗粒标记生物分子，并使用专利的超灵敏磁性传感器检测结合时产生的磁场。 虽然最初的重点将是直接检测两个主要 脓毒症的病原体，金黄色葡萄球菌和大肠杆菌，诊断平台 应普遍适用于广泛的病原体。 将通过测量磁性标记的结合来证明直接检测活细菌 针对目标病原体表面抗原的抗体。购买的细菌将首先用于 在快速的“混合和测量”磁性分析中展示高灵敏度的概念验证。然后，我们将生产下一代原型仪器，并将其放置在我们的合作者波士顿儿童医院。将对现有的IRB批准的记录感染的临床标本库(600份血液和尿液样本，150份气管抽吸物)进行检查，以证明其临床实用性，包括敏感性、特异性和较短的化验时间。 该程序的最终目标是在临床样本中显示检测极限&lt；100个细菌/毫升，总的(制备和测量)分析时间少于15分钟。此外，通过使用独特的能力利用磁场在外部操纵磁性纳米颗粒标签，可以在小样本(例如20微升)中检测到低至5-10个细菌。请注意，磁性检测不受生物流体中测量的阻碍，微板井中的混合和测量格式需要很少的样品准备，从而将误差降至最低。