Physiology of Single Presynaptic Terminals

描述（由申请人提供）： 本申请旨在支持Timothy Ryan博士（美国首席研究员）和Felipe Barros博士（外国合作者;智利）进行的合作研究。美国私家侦探长期以来，他一直对突触末梢中囊泡交通的调节以及这种交通如何影响突触功能感兴趣。目前的提议通过考虑突触活动如何与葡萄糖转运相匹配以及神经元及其周围星形胶质细胞之间如何分担代谢负荷来扩展他的研究范围。国外合作者Felipe Barros博士在代谢应激激活葡萄糖转运机制方面具有丰富经验，最近开发了基于共聚焦显微镜的方法，用于实时测量单个星形胶质细胞和神经元中的己糖转运。这项基于荧光成像的提议将联合收割机结合美国实验室在突触传递方面的专业知识和智利实验室在葡萄糖转运方面的专业知识。预计这项研究将为体内脑功能成像的分子机制提供新的线索。

DESCRIPTION (provided by applicant): This application is for support of collaborative research conducted by Dr. Timothy Ryan (U.S. Principal Investigator) and Dr. Felipe Barros (Foreign Collaborator; Chile). The U.S. P.I. has a long standing interest on the regulation of vesicle traffic in synaptic terminals, and how this traffic impacts synaptic function. The current proposal extends the breadth of his investigations by considering how synaptic activity is matched by glucose transport and how the metabolic load is shared between neurons and their surrounding astrocytes. The Foreign Collaborator, Dr. Felipe Barros, has experience on the mechanisms of glucose transport activation by metabolic stress and has recently developed methods based on confocal microscopy for realtime measurement of hexose transport in single astrocytes and neurons. The proposal, based on fluorescence imaging, will combine the know how of the US laboratory on synaptic transmission with the expertise of the Chilean laboratory on glucose transport. It is expected that this research will shed new light on the molecular mechanisms that underlie functional imaging of the brain in vivo.