High-Resolution SPECT for Molecular Imaging

用于分子成像的高分辨率 SPECT

• 批准号: 7111047
• 负责人: BENJAMIN M. W. TSUI
• 金额: $ 56.91万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-30 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7111047
• 关键词: X ray; atherosclerotic plaque; bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; computed axial tomography; genetically modified animals; image enhancement; image processing; laboratory mouse; molecular biology; phantom model; single photon emission computed tomography; technetium; three dimensional imaging /topography

DESCRIPTION (provided by applicant): The long-term goal of this project is to develop an advanced mu/SPECT/CT molecular imaging technology including a state-of-the-art innovative mu/SPECT system and accurate quantitative pinhole and multi-pinhole SPECT imaging techniques that provide ultra-high-resolution down to approximately 0.5 mm and detection efficiency on the order of 2 cps/mu/Ci. To evaluate the novel fSPECT/CT technology, we propose to perform simulations as well as experimental phantom and small animal imaging studies. To respond to the main concern of the reviewers that the previous proposal was too diffuse, we have refocused the research by redesigning the mu/SPECT system configuration with a fixed-angled detector assembly without going through the prototype stage, constructing the unique 'angled detector module assembly' with the latest high performance but robust detector technologies, eliminating the longitudinal tomography study using planar multi-pinhole imaging with standard gamma camera system, deleting two small animal imaging studies to focus on the most important application to imaging of plaques in mice, and switching to a simpler transgenic mouse model with stable and unstable plaques. Also, the proposal is strengthened by additional preliminary data from multi-pinhole image reconstruction, Monte Carlo simulation of pinhole SPECT data, and plaque imaging in transgenic mice using 99mTc Hynic-Annexin V. The team of investigators is streamlined with 2 groups of investigators and 3 key consultants with relevant areas of expertise. The revised specific aims are: (1) to develop a state-of-the-art 'angled' detector module and single and multiple pinhole collimation and an x-ray imaging device for use in the mu/SPECT/CT systems in Aim #5, (2) to develop simulation software for 3D SPECT imaging that models performance characteristics of the scintillation camera modules, design parameters of single and multiple pinhole collimator, and the SPECT system geometry and configuration, (3) to develop 3D quantitative SPECT image reconstruction methods for single and multiple pinhole SPECT with correction of attenuation, scatter and collimator response for accurate absolute quantitation of radioactivity in vivo, (4) to develop simple, accurate and robust calibration methods for single and multi-pinhole SPECT systems, (5) to develop a prototype mu/SPECT/CT system that includes two angled detector assemblies developed in Aim #1 and an existing mu/CT system that will be mounted on a commercial grade CT gantry, and (6) to evaluate the pinhole SPECT imaging techniques and the mu/SPECT/CT system in imaging stable and vulnerable plaques in a transgenic mouse model using 99mTc labeled Annexin-V.

描述（由申请人提供）： 该项目的长期目标是开发先进的mu/SPECT/CT分子成像技术，包括最先进的创新mu/SPECT系统和精确的定量针孔和多针孔SPECT成像技术，提供高达约0.5 mm的超高分辨率和2 cps/mu/Ci的检测效率。为了评估新的fSPECT/CT技术，我们建议进行模拟以及实验体模和小动物成像研究。为了回应评审人员的主要担忧，即之前的提案过于分散，我们重新调整了研究重点，重新设计了mu/SPECT系统配置，其中固定角度探测器组件没有经过原型阶段，使用最新的高性能但强大的探测器技术构建了独特的“角度探测器模块组件”，消除了使用具有标准伽马照相机系统的平面多针孔成像的纵向断层摄影研究，删除了两个小动物成像研究以集中于对小鼠中斑块成像的最重要应用，并切换到具有稳定和不稳定斑块的更简单的转基因小鼠模型。此外，该提案还得到了来自多针孔图像重建、针孔SPECT数据的蒙特卡罗模拟和使用99 mTc Hynic-Annexin V的转基因小鼠斑块成像的额外初步数据的加强。研究者团队由2组研究者和3名具有相关专业领域的关键顾问组成。修订后的具体目标是：（1）开发最先进的“成角度”探测器模块以及单针孔和多针孔准直器和X射线成像设备，用于目标#5中的mu/SPECT/CT系统，（2）开发用于3D SPECT成像的模拟软件，该模拟软件对闪烁照相机模块的性能特性、单针孔和多针孔准直器的设计参数以及SPECT系统的几何形状和配置进行建模，（3）开发用于单针孔和多针孔SPECT的3D定量SPECT图像重建方法，其具有衰减、散射和准直器响应的校正，用于体内放射性的精确绝对定量，（4）开发用于单针孔和多针孔SPECT系统的简单、精确和鲁棒的校准方法，（5）开发原型mu/SPECT/CT系统，该原型mu/SPECT/CT系统包括在目标#1中开发的两个成角度的检测器组件和将安装在商业级CT台架上的现有mu/CT系统，以及（6）评价针孔SPECT成像技术和mu/SPECT/CT系统在使用99 mTc标记的Annexin-V的转基因小鼠模型中对稳定和易损斑块的成像。