Approach to control caries through apple-derived vaccine

通过苹果疫苗控制龋齿的方法

• 批准号: 7135549
• 负责人: HILARY KOPROWSKI
• 金额: $ 15.14万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7135549
• 关键词: Agrobacterium; Streptococcus mutans; Streptococcus vaccine; active immunization; angiosperms; chimeric proteins; dental caries; dental caries vaccine; fruit; genetically modified plants; laboratory mouse; nonhuman therapy evaluation; oral administration; preventive dentistry; transfection /expression vector; vaccine development

DESCRIPTION: The goal of our research project is to produce safe, inexpensive and easily administered apple fruit-based vaccine which can be used in humans for immunization against Dental caries, (Streptococcus mutatis). We will accomplish this by developing transgenic apple (Malus domestica) producing the edible vaccine reagent. Specific Aims are to obtain plant binary expression vectors to carry chimeric protein consisting of the saliva-binding region (SBR) of S. mutans Agl/ll coupled to cholera toxin (CT) A2 and B subunits (CTA2B) and/or glucan-binding (GLU) protein of glucosyltransferases (GTF) for Agrobacterium-mediated transformation, to obtain transgenic apple line to express SBR-CTA2B and/or GLU genes. We will design the plant binary expression vector for Agrobacterium-mediated plant transformation to deliver SBR-CTA2B and/or GLU genes to apple genome. Hypotheses to be tested are that the SBR-CTA2B and/or GLU are expressed in transgenic apples and both SBR-CTA2B and GLU proteins expressed in apple synergistically elicits protective immune responses in mice. First, we will obtain transgenic apple plant to produce antigenic SBR-CTA2B and GLU proteins and quantify the amount of SBR-CTA2B and/or GLU proteins produced in in vitro transgenic apples. Protective immune response in mice will be observed in mice immunized by transgenic apple leaf producing SBR-CTA2B and/or GLU proteins. We will test if the SBR-CTA2B and/or GLU proteins expressed in transgenic apple leaf tissue induces serum and fecal IgG and IgA, and salivary IgA responses in mice. We also will investigate whether there is synergy between SBR-CTA2/B and GLU oral immunization with respect to protection to Dental caries in mice. The long range goal is to develop a potential and useful fruit-based delivery system for a vaccine against Dental caries. The proposed research will initiate a novel approach to develop a plant fruit-based vaccine delivery system for a pharmaceutical vaccine against Dental caries and other infectious diseases.

描述：我们的研究项目的目标是生产安全、廉价和易于管理的苹果水果疫苗，用于人体预防龋齿(变形链球菌)的免疫。我们将通过开发生产可食用疫苗试剂的转基因苹果(Malus Home Tica)来实现这一点。目的获得携带变形链球菌唾液结合区(SBR)与霍乱毒素(CT)A2和B亚基(CTA2B)和/或葡糖转移酶(GTF)葡聚糖结合蛋白(GLU)的嵌合蛋白的植物双元表达载体，用于农杆菌介导的转化，获得表达SBR-CTA2B和/或GLU基因的转基因苹果品系。我们将设计用于农杆菌介导植物转化的植物双元表达载体，将SBR-CTA2B和/或GLU基因转移到苹果基因组中。有待检验的假设是，在转基因苹果中表达了SBR-CTA2B和/或GLU，并且在苹果中表达的SBR-CTA2B和GLU蛋白都能协同诱导小鼠产生保护性免疫反应。首先，我们将获得转基因苹果植株，以产生抗原性的SBR-CTA2B和GLU蛋白，并对体外转基因苹果产生的SBR-CTA2B和/或GLU蛋白的量进行定量。用产生SBR-CTA2B和/或GLU蛋白的转基因苹果叶免疫小鼠，可观察到保护性免疫反应。我们将测试在转基因苹果叶组织中表达的SBR-CTA2B和/或GLU蛋白是否能诱导小鼠血清和粪便中的免疫球蛋白和免疫球蛋白A以及唾液中的免疫球蛋白A反应。我们还将研究SBR-CTA2/B和GLU口服免疫在预防小鼠龋齿方面是否存在协同作用。长期目标是开发一种潜在的、有用的水果递送系统，用于预防龋齿的疫苗。这项拟议的研究将启动一种新的方法，开发一种以植物水果为基础的疫苗递送系统，用于预防龋齿和其他传染病的药物疫苗。