Molecular Epidemiology of Human P. carinii Pneumonia

人卡氏疟原虫肺炎的分子流行病学

• 批准号: 7084609
• 负责人: LAURENCE HUANG
• 金额: $ 12.16万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7084609
• 关键词: B lymphocyte; HIV infections; Pneumocystis carinii; Pneumocystis pneumonia; T lymphocyte; cellular immunity; clinical research; communicable disease transmission; epidemiology; flow cytometry; fungal genetics; hospitals; human subject; patient oriented research; polymerase chain reaction; questionnaires; virus infection mechanism; western blottings

DESCRIPTION (provided by applicant): This is a resubmission of a K23 grant application that is designed to enable Dr. Laurence Huang to acquire additional clinical research training and to develop a research program that will allow him to become an independent and successful clinical investigator. The plan emphasizes didactic training in clinical epidemiology, molecular methods in clinical research, and advanced statistical analysis techniques, which he will gain through completion of a Master's Degree Program in Clinical Research. This didactic training will be coupled with the opportunity to spend time in the laboratories of his scientific collaborators so that he may gain practical insights into the new and highly promising molecular techniques he proposes to use to examine the epidemiology and transmission of human Pneumocystis carinii pneumonia. These studies will involve the close collaboration of scientists from the CDC, NIH, and several university-based laboratories. and will be conducted under the guidance of his two co-mentors: Philip Hopewell, MD, one of the world's foremost experts in the molecular epidemiology of tuberculosis, and Dennis Osmond, PhD, a leading HIV/AIDS epidemiologist and biostatistician. Although the transmission of Pneumocystis carinii between mammals of the same species has been convincingly demonstrated in the laboratory, it is unproven whether a similar clinical model exists for human disease. In addition, it is debated whether PCP in humans develops solely from reactivation or of a latent infection acquired early in childhood or also from acquisition of a new infection due to a recent exposure. The proposed research plan will determine whether P. carinii and immune responses to P. carinii can be detected in household and hospital contacts living with and caring for HIV-infected patients with PCP and, if P. carinii is detected, whether the P. carinii genetic sequence from a contact matches that from the patient with PCP. This information will be used to develop future studies to examine whether PCP can result from acquisition of a new infection. Since PCP remains a leading cause of the moribiity and mortality in HIV-infected and other immunocompromised persons, insights into its epidemiology and transmission may have profound implications for clinical management and disease prevention.

描述(由申请人提供)： 这是K23拨款申请的重新提交，该申请旨在使Laurence Huang博士能够获得额外的临床研究培训，并开发一个研究计划，使他能够成为一名独立和成功的临床研究员。该计划强调临床流行病学的教学培训，临床研究中的分子方法，以及先进的统计分析技术，他将通过完成临床研究硕士课程而获得这些技能。这一教学培训将伴随着在他的科学合作者的实验室中度过的机会，以便他可以对他建议用来检查人类卡氏肺孢子虫肺炎的流行病学和传播的新的和非常有前途的分子技术有实际的见解。这些研究将涉及来自疾控中心、美国国立卫生研究院和几个大学实验室的科学家的密切合作。并将在他的两位共同导师的指导下进行：世界上最顶尖的结核病分子流行病学专家之一菲利普·霍普韦尔医学博士和领先的艾滋病毒/艾滋病流行病学家和生物统计学家丹尼斯·奥斯蒙德博士。尽管在实验室中已经令人信服地证明了卡氏肺孢子虫在同类哺乳动物之间的传播，但人类疾病是否存在类似的临床模式仍未得到证实。此外，人们还在争论，人类的PCP是仅仅由于重新激活还是由于在童年早期获得的潜伏感染，还是也由于最近接触到的新感染而发展起来的。拟议的研究计划将确定是否可以在与卡氏肺囊虫肺炎患者一起生活和护理的艾滋病毒感染患者的家庭和医院接触者中检测到卡氏肺孢子虫和对卡氏肺孢子虫的免疫反应，如果检测到卡氏肺孢子虫，则确定接触者的卡氏肺孢子虫基因序列是否与PCP患者的卡氏肺孢子虫基因序列匹配。这些信息将被用来开展未来的研究，以检查新感染是否会导致PCP。由于PCP仍然是艾滋病毒感染者和其他免疫功能低下者死亡和死亡的主要原因，对其流行病学和传播的了解可能对临床管理和疾病预防具有深远的影响。

项目成果

A rose is a rose: but what about Pneumocystis pneumonia?

玫瑰就是玫瑰：但是肺孢子菌肺炎呢？

• 发表时间: 2005
• 期刊: The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease
• 作者: Huang,Laurence;Murray,JohnF
• 通讯作者: Murray,JohnF

Pneumocystis jirovecii infection.

耶氏肺孢子虫感染。

• DOI: 10.1136/thx.2004.021436
• 发表时间: 2004
• 期刊: Thorax
• 影响因子: 10
• 作者: Miller,R;Huang,L
• 通讯作者: Huang,L

Application of an mRNA-based molecular viability assay to oropharyngeal washes for the diagnosis of Pneumocystis pneumonia in HIV-infected patients. A pilot study.

基于 mRNA 的分子活力测定在口咽冲洗液中的应用，用于诊断 HIV 感染患者的肺孢子菌肺炎。

• DOI: 10.1111/j.1550-7408.2003.tb00652.x
• 发表时间: 2003
• 期刊: The Journal of eukaryotic microbiology
• 作者: Huang,Laurence;Crothers,Kristina;DeOliveira,Ana;Eiser,Shary;Zucchi,Patrizia;Beard,CharlesB;Unnasch,ThomasR
• 通讯作者: Unnasch,ThomasR

Dihydropteroate synthase gene mutations in Pneumocystis and sulfa resistance.

• DOI: 10.3201/eid1010.030994
• 发表时间: 2004-10
• 期刊: Emerging infectious diseases
• 影响因子: 11.8
• 作者: Huang L;Crothers K;Atzori C;Benfield T;Miller R;Rabodonirina M;Helweg-Larsen J
• 通讯作者: Helweg-Larsen J

Current epidemiology of Pneumocystis pneumonia.

• DOI: 10.3201/eid1010.030985
• 发表时间: 2004-10
• 期刊: Emerging infectious diseases
• 影响因子: 11.8
• 作者: Morris A;Lundgren JD;Masur H;Walzer PD;Hanson DL;Frederick T;Huang L;Beard CB;Kaplan JE
• 通讯作者: Kaplan JE