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Mining the untapped chemical potential of entomopathogenic fungi for sustainable agriculture and human health.

挖掘昆虫病原真菌未开发的化学潜力，促进可持续农业和人类健康。

基本信息

批准号：
2725900
负责人：
金额：
--
依托单位：
University of Greenwich
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2022
资助国家：
英国
起止时间：
2022 至无数据
项目状态：
未结题

来源：
https://gtr.ukri.org/projects?ref=studentship-2725900
关键词：
Mining untapped chemical potential entomopathogenic

项目摘要

Entomopathogenic fungi (EPF) are fungi that infect and kill insects. Some EPF havebeen developed as commercial biopesticides, the use of which has grown inimportance in response to the environmental impact of synthetic pesticides, changesin regulations (e.g. EU Directive 2009/128/EC), insecticide resistance and growingpublic demand for sustainably produced food. Existing research on the secondarymetabolites of EPF has focused on identifying compounds with medicinal benefits e.g.cyclosporine from Tolypocladium inflatum. However, this research has been hindereddue to EPF metabolites being produced transiently, in low quantities or not at all underlaboratory conditions. Goals: Develop new liquid fermentation methodologies toreplicate the biochemical conditions in insect hosts. Analyse differences in secondarymetabolite profile, compared to existing cultivation methods. Isolation and structuralelucidation of metabolites associated with pathogenesis, informing our knowledge ofthe mode of action and biosynthetic pathways. Using the larger scale liquid EPFcultures, bioprospect for novel compounds with drug or pest control properties usingan assay led methodology. Perform genome sequencing of one or more selectedisolates to profile total metabolite diversity within the pathogen genome. PerformRNA seq analysis of fermenter cultures to identify genes responsible for secondarymetabolite production. Use comparative genomics with publicly available EPFgenomes to identify conservation of key metabolite gene clusters with potential forpest control.

昆虫病原真菌（Entomopathogenic fungi，EPF）是一类能感染并杀死昆虫的真菌。一些EPF已被开发为商业生物农药，其使用已变得越来越重要，以应对合成农药的环境影响，法规的变化（例如欧盟指令2009/128/EC），杀虫剂抗性和公众对可持续生产食品的需求日益增长。对EPF次级代谢产物的现有研究集中在鉴定具有药用益处的化合物，例如来自Tolypocladium inflatum的环孢菌素。然而，由于EPF代谢产物在实验室条件下瞬时产生、数量少或根本不产生，这一研究一直受到阻碍。目的：开发新的液体发酵方法，以复制昆虫宿主的生化条件。与现有的培养方法相比，分析次级代谢产物谱的差异。与发病机制相关的代谢物的分离和结构阐明，为我们了解作用模式和生物合成途径提供信息。使用更大规模的液体EPF培养物，使用分析方法对具有药物或害虫控制特性的新型化合物进行生物展望。对一种或多种选定的代谢物进行基因组测序，以分析病原体基因组内总代谢物的多样性。对发酵罐培养物进行RNA测序分析，以确定负责次级代谢产物产生的基因。使用比较基因组学与公开的EPF基因组，以确定保护的关键代谢物基因簇与潜在的害虫控制。