Islet culture, shipping, and infusion device

胰岛培养、运输和输注装置

• 批准号: 7114785
• 负责人: John Wilson
• 金额: $ 6.25万
• 依托单位: WILSON WOLF MANUFACTURING CORPORATION
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-30 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7114785
• 关键词: animal tissue; biomedical equipment development; clinical research; consumable /disposable biomedical equipment; gas; growth media; human tissue; injection /infusion; insulin dependent diabetes mellitus; membrane permeability; miniature biomedical equipment; oxygen consumption; packaging material; pancreatic islet transplantation; pancreatic islets; tissue /cell culture

DESCRIPTION (provided by applicant): There exists a need for a single device that can culture, ship, and infuse islet. Flasks and petri dishes are the devices currently used to store islets. They are a weak link in the process because these devices have inherent design limitations that do not allow them to maintain islets at high viability during culture or shipping. The problem is the need to oxygenate islets by a gas/medium interface. The net result is that many devices are required for a single transplant, the process is not compatible with regulatory control, and precious islets are lost due to necrosis. There are no commercially available substitutes that offer a superior alternative. This grant is focuses upon creating a gas permeable device that cultures, ships, and infuses islets at high viability in a manner compatible with regulatory control. This device will be superior to any commercially available alternatives. By eliminating the link between oxygen delivery and a gas/medium interface, a device that allows high density culture at high viability becomes available. The islets are at improved oxygen tension, with extra medium capacity, from culture through shipping. Primary and backup device configurations are proposed. One configuration is based on culturing islets at high density in an extremely easy to use device. A backup device integrates a high amount of surface area to house islets at a wide range of possible densities. Aim 1 determines which configuration will be pursued, and creates its design specifications. Of primary interest is the optimal gas permeable surface area to medium volume ratios that indicate device size and shape. Aim 2 produces small scale prototypes of the device and quantifies the performance relative to the flask using porcine and human islets, and provides design specifications for Aim 3. Aim 3 produces full scale device prototypes and quantifies the performance relative to the flask using porcine and human islets. If the novel device proves superior to the flask, specifications of the device will be delivered to Phase II which will investigate making the device suitable for shipping and infusing islets.

描述(由申请人提供)： 需要一种能够培养、运输和注入胰岛的单一设备。烧瓶和培养皿是目前用来储存胰岛的设备。它们是这一过程中的薄弱环节，因为这些设备有固有的设计限制，不允许它们在培养或运输期间保持高活性的胰岛。问题是需要通过气体/介质界面给胰岛充氧。最终的结果是，一次移植需要许多设备，过程与监管控制不兼容，宝贵的胰岛因坏死而丢失。没有商业上可用的替代品可以提供更好的替代品。 这笔赠款的重点是创造一种气体渗透装置，以符合监管控制的方式培养、运输和注入具有高生存能力的胰岛。这种设备将优于任何商业上可用的替代品。通过消除氧气输送和气体/介质界面之间的联系，可以在高活力的情况下进行高密度培养的设备成为可能。从培养到运输，这些胰岛的氧分压都得到了改善，有了额外的介质容量。提出了主设备和备份设备配置。一种配置是基于在极易使用的设备中以高密度培养胰岛。备份装置集成了大量的表面积以容纳各种可能密度的小岛。目标1确定将采用哪种配置，并制定其设计规范。主要关注的是表明设备大小和形状的最佳气体渗透表面积与介质体积比。AIM 2生产该装置的小规模原型，并使用猪胰岛和人胰岛量化相对于烧瓶的性能，并为AIM 3提供设计规范。AIM 3生产完整尺寸的设备原型，并使用猪岛和人胰岛量化相对于烧瓶的性能。如果新的设备被证明优于烧瓶，设备的规格将被提交到第二阶段，该阶段将调查该设备是否适合运输和输注胰岛。

项目成果

Assessment of tissue-engineered islet graft viability by fluorine magnetic resonance spectroscopy.

通过氟磁共振波谱评估组织工程胰岛移植物的活力。

• DOI: 10.1016/j.transproceed.2011.09.009
• 发表时间: 2011
• 期刊: Transplantation proceedings
• 影响因子: 0.9
• 作者: Suszynski,TM;Avgoustiniatos,ES;Stein,SA;Falde,EJ;Hammer,BE;Papas,KK
• 通讯作者: Papas,KK

Islet preparation purity is overestimated, and less pure fractions have lower post-culture viability before clinical allotransplantation.

• DOI: 10.1016/j.transproceed.2014.06.011
• 发表时间: 2014-07
• 期刊: Transplantation proceedings
• 影响因子: 0.9
• 作者: Kitzmann JP;Karatzas T;Mueller KR;Avgoustiniatos ES;Gruessner AC;Balamurugan AN;Bellin MD;Hering BJ;Papas KK
• 通讯作者: Papas KK

Metabolic profile of pancreatic acinar and islet tissue in culture.

培养物中胰腺腺泡和胰岛组织的代谢特征。

• DOI: 10.1016/j.transproceed.2014.06.003
• 发表时间: 2014
• 期刊: Transplantation proceedings
• 影响因子: 0.9
• 作者: Suszynski,TM;Mueller,KR;Gruessner,AC;Papas,KK
• 通讯作者: Papas,KK

Manufacturing porcine islets: culture at 22ýýýýC has no advantage above culture at 37ýýýýC: a gene expression evaluation.

制造猪胰岛：22°C 培养比 37°C 培养没有优势：基因表达评估。

• DOI: 10.1111/xen.12048
• 发表时间: 2013
• 期刊: Xenotransplantation
• 影响因子: 3.9
• 作者: Mueller,KateR;Martins,KyraV;Murtaugh,MichaelP;Schuurman,Henk-Jan;Papas,KlearchosK
• 通讯作者: Papas,KlearchosK