Superior cell culture device for more efficient biological production

卓越的细胞培养装置，实现更高效的生物生产

• 批准号: 7910018
• 负责人: John Wilson
• 金额: $ 11.35万
• 依托单位: WILSON WOLF MANUFACTURING CORPORATION
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-10 至 2014-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7910018
• 关键词: Area; Biological; Biotechnology; Cell Culture Techniques; Cells; Confidential Information; Databases; Devices; Dialysis procedure; Equipment; Evaluation; Goals; Growth Factor; Laboratories; Lead; Membrane; Mesenchymal; Paper; Phase; Process; Production; Proteins; Recombinant Proteins; Recovery; Relative (related person); Reporting; Research; Resources; Roller Bottle; Savings; Shapes; Sterility; Surface; Testing; Time; Viral Vector; Work; cost; density; design; flasks; performance tests; public health relevance; wasting

DESCRIPTION (provided by applicant): For much of the small and mid-scale cell culture work at the core of biotechnology, traditional culture ware such as flasks and roller bottles are the only devices that are practical for use. Although they are easy to use and disposable, they have design limits that cause them to squander labor, laboratory space, disposal space, growth factors, protein recovery materials, and time. This application proposes a new cell culture device that is far superior to traditional culture ware. The new device is called the "6X Membrane Roller Bottle (6X MRB)." It has an outside shape that takes the shape of a roller bottle so that it can interface with standard lab equipment. However, it has a unique internal geometry that allows it to grow more cells in less space, concentrate cell secreted protein, and reduce the use of growth factors. Millions of dollars of savings are projected annually. While the design concept appears strong on paper, feasibility needs to be proven through biological performance testing. Thus, the primary goal of Phase 1 is to determine if the 6X MRB concept will perform as hypothesized when faced with actual biological evaluations. Specific aims follow. Aim 1: Fabricate 6X MRB test devices in accordance with our concept design. A six fold increase in surface area will be present relative to traditional culture ware and a dialysis membrane will allow cells to be cultured at high density. Deliverable: Sterile 6X MRB test devices ready for Aim 2 testing. Aim 2: Determine feasibility of the 6X MRB by conducting a biological assessments with BHK cells secreting EPO, mesenchymal cells using expensive growth factors, and PG13 cells secreting viral vectors to determine its capacity to cost reduce the cell culture process relative to traditional culture ware. Deliverable: Report whether or not Phase II is warranted. If warranted, a design data base will be available to guide the Phase II plan. PUBLIC HEALTH RELEVANCE: For much of the small and mid-scale cell culture work at the core of biotechnology, traditional culture ware such as flasks and roller bottles are the only devices that are practical for use. Although they are easy to use and disposable, they squander labor, laboratory space, disposal space, growth factors, protein recovery materials, and time. In the absence of a superior device alternative, a significant portion of the vast amount of money spent on cell culture is wasted. A new cell culture device is proposed that overcomes these problems and is expected to allow millions of dollars annually to be redirected from mundane cell culture activity to much more important aspects of research.

描述（由申请人提供）：对于生物技术核心的许多中小型细胞培养工作，烧瓶和滚瓶等传统培养器皿是唯一实用的设备。尽管它们易于使用且一次性，但它们的设计限制导致它们浪费劳动力、实验室空间、处置空间、生长因子、蛋白质回收材料和时间。该申请提出了一种远远优于传统培养器皿的新型细胞培养装置。新设备被称为“6X 膜滚瓶（6X MRB）”。它的外部形状呈滚瓶形状，因此可以与标准实验室设备连接。然而，它具有独特的内部几何形状，使其能够在更小的空间内生长更多的细胞，浓缩细胞分泌的蛋白质，并减少生长因子的使用。预计每年可节省数百万美元。虽然设计理念在纸面上看起来很强大，但可行性需要通过生物性能测试来证明。因此，第一阶段的主要目标是确定 6X MRB 概念在面对实际生物学评估时是否会像假设的那样发挥作用。具体目标如下。目标 1：根据我们的概念设计制造 6X MRB 测试设备。相对于传统培养器皿，表面积将增加六倍，并且透析膜将允许细胞以高密度培养。 可交付成果：无菌 6X MRB 测试设备已准备好进行 Aim 2 测试。目标 2：通过对分泌 EPO 的 BHK 细胞、使用昂贵的生长因子的间充质细胞和分泌病毒载体的 PG13 细胞进行生物学评估，确定 6X MRB 的可行性，以确定其相对于传统培养容器降低细胞培养过程成本的能力。 可交付成果：报告第二阶段是否有必要。 如果有必要，将提供设计数据库来指导第二阶段计划。 公共健康相关性：对于生物技术核心的许多中小型细胞培养工作来说，烧瓶和滚瓶等传统培养器具是唯一实用的设备。尽管它们易于使用且一次性，但它们浪费劳动力、实验室空间、处置空间、生长因子、蛋白质回收材料和时间。如果没有更好的设备替代方案，花在细胞培养上的巨额资金中很大一部分就会被浪费。提出了一种新的细胞培养装置来克服这些问题，预计每年可以将数百万美元从普通的细胞培养活动转移到更重要的研究方面。