Islet culture, shipping, and infusion device
胰岛培养、运输和输注装置
基本信息
- 批准号:7406168
- 负责人:
- 金额:$ 110.94万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2004
- 资助国家:美国
- 起止时间:2004-09-30 至 2011-03-31
- 项目状态:已结题
- 来源:
- 关键词:AddressCharacteristicsCommunitiesCultured CellsDevicesDiabetes MellitusElectronicsGasesGrantHealth Care CostsIncubatorsInfusion proceduresInvestigationIslets of LangerhansMarketingMethodsMolecular ProfilingOutcomeOxygen ConsumptionPositioning AttributePreparationProcessProtocols documentationQuality of lifeRateRelative (related person)ResearchRoleShippingShipsSocietiesTechniquesTissuesTransfusionTransplantationdaydensitydesignfeedingflasksimmunogenicityisletnovel
项目摘要
DESCRIPTION (provided by applicant): The culture and shipment of islets are an integral part of islet transplant research. Flasks, petri dishes, and bags are the devices used to perform that role. However, these devices have inherent design characteristics that make them poorly suited to the unique needs of islet culture and islet shipping. This results in the use of far too many devices to maintain an islet preparation, a high potential for contamination, severely limited process control, and islet damage during shipping. Unfortunately, little effort has been undertaken to address the problems inherent to existing devices. We propose the creation of a superior device that is ideally suited to oxygenate islets and is tailored to the needs of the islet community. It will be the only device capable of culturing, shipping, and infusing an entire pancreatic islet isolation. This device is referred to as the single layer gas permeable device (SLD). Our investigation is expected to achieve five specific aims. Aim 1 will define a high-density SLD islet culture protocol that provides islets of superior quality and less immunogenicity than possible with existing devices when an entire islet preparation is cultured in a single device for at least seven-days without feeding. Outcomes will be determined by a variety of techniques including oxygen consumption rate (OCR), OCR/DNA, and molecular profiling. Aim 2 will adapt the SLD from a culture to a shipping device. Aim 3 will create a novel gyroscopic shipping container (GSC) that functions as an incubator without need of electronic controls and maintains the SLD in a standing position independent of how its outer container is oriented during shipping. This essentially allows islets to be cultured during shipping. Aim 4 will verify that the SLD of Aim 2 and the GSC of Aim 3 deliver islet tissue with superior viable islet yield, quality, and reduced immunogenicity relative to existing methods. Aim 5 will modify the SLD for islet transfusion and verify the superiority of the SLD for islet culture, shipping, and infusion relative to existing methods.
Project Narrative: Islet transplants hold promise to provide a better quality of life for those afflicted with diabetes and reduce health care cost to society. There are no cell culture devices on the market that are designed with the attributes needed to efficiently oxygenate islets during culture and shipping. This grant is intended to create a gas permeable device (SLD) that can culture, ship, and infuse islets with maximum efficiency.
描述(由申请人提供):胰岛的培养和运输是胰岛移植研究的组成部分。烧瓶、培养皿和袋子是用于执行该角色的设备。然而,这些装置具有固有的设计特征,使得它们不太适合胰岛培养和胰岛运输的独特需求。这导致使用太多的装置来维持胰岛制备、高污染可能性、严重受限的过程控制以及运输期间的胰岛损伤。不幸的是,几乎没有做出努力来解决现有设备固有的问题。我们建议创建一个上级设备,非常适合胰岛,并根据胰岛社区的需求量身定制。它将是唯一能够培养、运输和输注整个胰岛分离物的装置。该装置被称为单层透气装置(SLD)。我们的调查预期达到五个具体目标。目标1将定义一种高密度SLD胰岛培养方案,当整个胰岛制剂在单个装置中培养至少七天而不喂食时,该方案提供的胰岛比现有装置具有更高的上级质量和更低的免疫原性。结果将通过各种技术确定,包括耗氧率(OCR),OCR/DNA和分子分析。目标2将使SLD从培养物适应运输器械。目标3将创建一种新型的陀螺仪运输容器(GSC),其用作培养箱,而无需电子控制,并将SLD保持在站立位置,而不受其外部容器在运输过程中如何定向的影响。这基本上允许在运输期间培养胰岛。目的4将验证目的2的SLD和目的3的GSC递送相对于现有方法具有上级活胰岛产量、质量和降低的免疫原性的胰岛组织。目的5将修改用于胰岛输注的SLD,并验证SLD相对于现有方法用于胰岛培养、运输和输注的优越性。
项目叙述:胰岛移植有望为糖尿病患者提供更好的生活质量,并降低社会的医疗成本。市场上没有设计有在培养和运输过程中有效培养胰岛所需属性的细胞培养装置。该补助金旨在创造一种透气装置(SLD),可以最大限度地培养,运输和注入胰岛。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(1)
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John Wilson其他文献
John Wilson的其他文献
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