Neuropathology and Tissue Culture Core

神经病理学和组织培养核心

• 批准号: 6919799
• 负责人: Luis Del Valle
• 金额: $ 22.97万
• 依托单位: TEMPLE UNIV OF THE COMMONWEALTH
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6919799
• 关键词: AIDS; AIDS /HIV neuropathy; AIDS dementia complex; Polyomavirus hominis 2; biomedical facility; embryo /fetus cell /tissue; genetically modified animals; human immunodeficiency virus 1; human tissue; laboratory mouse; laboratory rat; neuropathology; progressive multifocal leukoencephalopathy; tissue /cell culture; tissue /cell preparation; tissue resource /registry; viral myelinopathy; virus virus interaction

This proposal seeks continued funding of the Neuropathology and Tissue Culture Core to assist the overall program project grant in evaluating the pathogenesis of HIV-1-associated dementia and Progressive Multifocal Leukoencephalopathy (PML) and the cooperative molecular mechanisms through which HIV-1 and JC virus interact. We will provide neuropathological expertise fore the evaluation of surgical and autopsy brain tissue samples from AIDS and non-AIDS PML patients with HIV-1 encephalopathy and control tissue to complement molecular studies performed in Projects 1,2, and 3. We will provide highly purified cultures of microglia, oligodendrocytes, and astrocytes from human fetal CNS tissue as needed for the individual projects. The cultures will undergo quality control testing to ensure uniform distribution across the projects. We will provide cultures of established permanent cell lines, including the progenitor CG-4 cells and mouse embryo fibroblasts from Pur-alpha knockout mice as a service to all three projects. We will also offer on demand culturing of primary cells or establishment of cell lines from rats and genetically modified mice. The proposed Core services will provide essential and uniform material to the three participant projects to assist in their studies on the molecular biology of HIV-1 and JCV interaction with host cellular factors and will contribute to our understanding of the pathogenesis of these two CNS disorders.

该提案寻求继续资助神经病理学和组织培养核心，以协助整体计划项目赠款评估HIV-1相关痴呆和进行性多灶性白质脑病（PML）的发病机制以及HIV-1和JC病毒相互作用的合作分子机制。我们将提供神经病理学的专业知识，以评估来自艾滋病和非艾滋病PML患者的手术和尸检脑组织样本与HIV-1脑病和对照组织，以补充在项目1，2和3中进行的分子研究。我们将根据个别项目的需要，提供来自人胎儿CNS组织的小胶质细胞、少突胶质细胞和星形胶质细胞的高度纯化培养物。将对培养物进行质量控制检测，以确保在项目中均匀分布。我们将提供已建立的永久细胞系的培养物，包括祖细胞CG-4细胞和来自Pur-alpha敲除小鼠的小鼠胚胎成纤维细胞，作为对所有三个项目的服务。我们还将根据需要提供原代细胞培养或从大鼠和转基因小鼠建立细胞系。拟议的核心服务将为三个参与项目提供必要和统一的材料，以协助他们研究HIV-1和JCV与宿主细胞因子相互作用的分子生物学，并将有助于我们了解这两种中枢神经系统疾病的发病机制。