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Molecular Histopathology and Analytical Microscopy Core

分子组织病理学和分析显微镜核心

基本信息

批准号：
10664023
负责人：
Luis Del Valle
金额：
$ 15.11万
依托单位：
LSU HEALTH SCIENCES CENTER
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-08-15 至 2027-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10664023
关键词：
Animal Experiments Animals Antibodies Applications Grants Archives Autopsy Biological Assay Biopsy Specimen Board Certification Cell Culture Techniques Cell model Cell surface Cells Cellular Structures Centers of Research Excellence Clinical Collaborations Collection Comet Assay Confocal Microscopy DNA DNA Probes Data Dedications Detection Development Evaluation Extramural Activities Funding Generations Genes Grant HIV Histologic Histological Techniques Histology Histopathology Human Image Image Analysis Imaging Techniques Immunofluorescence Immunologic Immunohistochemistry In Situ In Situ Hybridization In Vitro Individual Infection Label Ligation Louisiana Malignant Neoplasms Manuscripts Mentors Messenger RNA Methodology Methods Microscope Microscopic Microscopy Mission Molecular NCI Center for Cancer Research Neoplasms Normal tissue morphology Oncology Pathologist Pathology Pathway interactions Patients Phase Photography Principal Investigator Process Protein Dynamics Proteins RNA RNA Probes RNA-Protein Interaction Reproducibility Research Research Activity Research Personnel Research Project Grants Research Support Sampling Services Technical Expertise Techniques Tissues Training Translational Research Tumor Tissue United States National Institutes of Health Universities Viral Viral Proteins Work biobank cancer cell data submission design detection method digital experience fluorescence microscope health organization human disease imaging study imaging system instrumentation nature center neoplastic programs prospective protein expression tissue processing tumor tumor registry virus related cancer

项目摘要

ABSTRACT The Molecular Histopathology and Analytical Microscopy Core (MHAM) is a state-of-the- art facility, created to provide advanced histopathology and microscopy services to researchers and clinicians conducting research at LSUHSC, including promising junior principal investigators (JPIs) included in the second phase of the Center for Translational Viral Oncology (CTVO). The MHAM provides investigators with access to archival clinical samples, experimental planning design and imaging studies on the histology and pathology of tissues. MHAM can assist Phase 2 CTVO JPIs in assessing the expression of cell surface and intracellular molecules including viral proteins, using bright field, and confocal microscopy, immunohistochemistry, immunohistofluorescence, multiplexing, in situ hybridization, and in situ proximity ligation (DuoLink). The various methods are also used to determine protein and mRNA levels, their subcellular localization, protein-protein and RNA-protein interactions in the cells and tissues. Given the translational nature of the CTVO, this Core is led by a trained pathologist, Dr. Luis Del Valle who, in addition to offering imaging services, also provides histopathological information and evaluation on patient tissues. The MHAM, works in close collaboration with the new HIV/Clinical Tumor Biorepository (HCTB), the Department of Pathology, the Louisiana Tumor registry and different to provide Phase 2 CTVO investigators with access to clinical samples, which are crucial to investigate the molecular and cellular pathways in virus-induced cancers. Moreover, the MHAM core assists investigators to develop the necessary imaging and data for the submission of manuscripts and grants.

抽象的分子组织病理学和分析显微镜核心 (MHAM) 是最先进的艺术设施，旨在为研究人员提供先进的组织病理学和显微镜服务和在 LSUHSC 进行研究的临床医生，包括有前途的初级首席研究员（JPI）纳入转化病毒肿瘤学中心（CTVO）第二阶段。这 MHAM 为研究人员提供档案临床样本、实验计划的访问权限组织的组织学和病理学的设计和成像研究。 MHAM 可以协助第二阶段 CTVO JPI 评估细胞表面和细胞内分子（包括病毒）的表达蛋白质，使用明视野和共聚焦显微镜，免疫组织化学，免疫组织荧光、多重检测、原位杂交和原位邻近连接（双联）。各种方法也用于测定蛋白质和 mRNA 水平，它们的细胞和组织中的亚细胞定位、蛋白质-蛋白质和RNA-蛋白质相互作用。鉴于 CTVO 的转化性质，该核心由训练有素的病理学家 Luis Del 博士领导 Valle 除了提供影像服务外，还提供组织病理学信息以及对患者组织的评估。 MHAM 与新的 HIV/临床肿瘤生物储存库 (HCTB)，路易斯安那州肿瘤病理学系注册中心和不同之处在于为第二阶段 CTVO 研究人员提供临床样本的访问权限，这对于研究病毒诱导的癌症的分子和细胞途径至关重要。此外，MHAM 核心还协助研究人员开发必要的成像和数据提交手稿和资助。