Molecular Histopathology Analytical Microscopy Core (MHAM)

摘要 分子组织病理学和分析显微镜核心(MHAM)是为了提供先进的 为LSUHSC进行研究的研究人员和临床医生提供组织病理学和显微镜服务， 包括翻译病毒肿瘤学中心(CTVO)有前途的初级调查人员(PJI)。这个 MHAM为PJIS提供组织组织学的实验规划设计和成像研究， 使用最先进的BREAT技术表达细胞表面和细胞内的大分子，包括病毒蛋白 现场、共聚焦显微镜、免疫组织化学、免疫组织化学荧光、原位杂交和 原位近距离结扎术(DuoLink)。这些方法也被用来确定蛋白质和信使核糖核酸水平， 它们在细胞和组织中的亚细胞定位、蛋白质-蛋白质和RNA-蛋白质相互作用。给定 CTVO的翻译性质，这个核心由训练有素的病理学家Luis Del Valle博士领导，他除了 提供影像服务，还提供患者组织的组织病理学信息和评估。这个 MHAM与新的艾滋病毒/临床肿瘤生物库(HCTB)核心密切合作，将提供 PJI可以获得临床样本，这些样本对于研究病毒的分子和细胞途径至关重要- 诱发癌症。此外，MHAM核心为调查人员提供组织处理和 管理并协助调查人员开发必要的图像和数据以提交 手稿和赠款。

ABSTRACT The Molecular Histopathology and Analytical Microscopy Core (MHAM) was created to provide advanced histopathology and microscopy services to researchers and clinicians conducting research at LSUHSC, including promising junior investigators (PJIs) of the Center for Translational Viral Oncology (CTVO). The MHAM provides PJIs with experimental planning design and imaging studies on the histology of tissues, the expression of cell surface and intracellular macromolecules including viral proteins, using state-of-the-art bright field, and confocal microscopy, immunohistochemistry, immunohistofluorescence, in situ hybridization, and in situ proximity ligation (DuoLink). These multiple methods are also used to determine protein and mRNA levels, their subcellular localization, protein-protein and RNA-protein interactions in the cells and tissues. Given the translational nature of the CTVO, this core is led by a trained pathologist, Dr. Luis Del Valle who, in addition to offering imaging services, also provides histopathological information and evaluation on patient tissues. The MHAM, working in close collaboration with the new HIV/Clinical Tumor Biorepository (HCTB) core will provide PJI's with access to clinical samples that are crucial to investigate the molecular and cellular pathways in virus- induced cancers. Moreover, the MHAM core provides investigators with training on tissue processing and management and assists investigators develop the necessary imaging and data for the submission of manuscripts and grants.