Core--Neuropathology and tissue culture

神经病理学和组织培养核心设施的中心作用是协助 本计划项目的目的是确定HIV-1脑病发展中涉及的不同分子途径。核心将为该计划项目的参与者提供来自诊断为HIV- 1脑病的HIV-1感染患者的手术和尸检脑组织材料，以及来自无CNS受累的HIV-1感染者和正常对照脑的脑组织材料。与项目1、2和3密切合作，我们将进行组织学和免疫组织化学分析，包括病毒蛋白、细胞标志物和各种细胞调节因子的双标记。神经病理学和组织培养核心设施将根据需要提供高度纯化的人胎儿小胶质细胞，星形胶质细胞和神经元培养物。神经元的原代培养物也将从大鼠 和小鼠海马体进行研究。将对已建立的细胞系PC 12和P19进行维护、培养并分发给项目。此外，还将制备来自HIV-1感染细胞的条件培养基，供各个项目使用。通过这一科学核心，我们将确保将必要资源有效分配给所有三个项目，并在保持最高水平的同时统一评估组织学和免疫组化数据 质量控制。

The central role of the Neuropathology and Tissue Culture Core Facility is to assist the overall objectives of this Program Project to determine the different molecular pathways involved in the development of HIV-1 Encephalopathy. The Core will furnish the participants of the Program Project with surgical and autopsy brain tissue material from HIV-1 infected patients diagnosed with HIV- 1 Encephalopathy, as well as brains from HIV-1 infected individuals with no CNS involvement and normal control brains. In close collaboration with Projects 1,2 and 3, we will perform histological and immunohistochemical analysis, including double labeling, of viral proteins, cellular markers, and various cellular regulatory factors. The Neuropathology and Tissue Culture Core Facility will provide highly purified cultures of human fetal microglia, astrocytes, and neurons on demand. Primary cultures of neurons will also be prepared from rat and mouse hippocampus for the proposed studies. The established cell lines, PC12 and P19 will be maintained, cultured, and distributed to the projects. Also, Conditioned Media from HIV-1 infected cells will be prepared for use by the individual projects. Through this scientific core, we will ensure the efficient distribution of essential resources to all three projects and uniform evaluation of histological and immunohistochemical data while maintaining the highest level of quality control.